SABEU GmbH & Co. KG

With our cellQART® Cell Culture Inserts we are dedicated to resolve the dilemma of customer needs for innovative cell culture products meeting highest standards at affordable prices.

This product portfolio will come with PET membranes in translucent or clear optics and 6-, 12- or 24-Well format which is compatible with most standard Cell Culture Plates. Our standard portfolio includes pore sizes from 0.4 μm to 8.0 μm with various pore densities.

Project based customization (especially alternative membranes, e.g. black colored or further pore sizes) is possible due to 100 % in-house production of plastics & membrane components.

All Inserts are TC treated and gamma sterilized in general as well as RNase / DNase free and non-pyrogenic. With our industry leading quality standards we ensure 100 % membrane parameter consistency for reproducible cell culture results.

Benefits:

• Affordable prices – buy directly from the true OEM
• Unique 100 % membrane parameter consistency for reproducible cell culture results
• Broad standard portfolio and customization – Ensured by complete in-house plastics & membrane production

Using the membrane filter method, a sample is filtered through a TRAKETCH® membrane. All microorganisms in a 100 ml sample solution are concentrated and retained completely (100%) on the membrane surface.

Detection is performed directly on the membrane by adding a fluorescent dye which can only penetrate through intact cell walls (living organisms). To ensure adequate measurement of the emitted fluorescence after stimulation by a laser, the membrane should have a low fluorescence. Otherwise we would see "a white image on a white background", i.e. we would only see a fluorescence of the entire surface.

To avoid this, SABEU stains this membrane in deep black to quench the fluorescence in defined ranges of wavelengths. This entire microbiological detection method is called solid phase cytometry, in which the membrane represents the solid phase. This method is not only rapid, but also in real time, i.e. within just 1–2 hours. In comparison, the growth of microcolonies takes 1–2 days, whereas growing visible colonies takes 1–2 weeks, depending on the microorganism.