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BioTeZ Berlin-Buch GmbH
Robert-Rössle-Str. 10,
13125
Berlin
Germany
Germany
This company is co-exhibitor of
DiagnostikNet-BB Netzwerk Diagnostik Berlin-Brandenburg e.V.
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18.11.2019
Topic
all-day
Demonstration of the TAPAS Lable
BioTeZ established a novel surface functionalization kit -the TAPAS system.
The strong interaction between streptavidin and biotin is widely utilized in biotechnological applications (1). The new TAPAS label was developed to extend the portfolio for assay manufactured and will be valuable for its applications across a wide range of biotechnological areas.
To meet the big demand BioTeZ introduced in 2008 the poly-streptavidin a crosslinked streptavidin polymer with increased biotin binding capacity widely used for lateral flow test applications (2). Now is 2018 BioTeZ released a novel surface functionalization platform the TAPAS Labeling Kit. This kit or labeling service allows an effective labeling a target molecules and detection with specific antibody.
The BioTeZ TAPAS Labeling Kit was developed to extend the portfolio for assay manufactured and will be valuable for its applications across a wide range of areas.The BioTeZ TAPAS Labeling Kit is a two component protein-ligand interaction system. One component is the small 1000 Dalton synthetic label called “TA-label”.
The second component is the “PAS-Antibody” which binds the “TA-label” with high specificity and efficiency. The BioTeZ TAPAS Labeling Kit or labelling service allows antibodies, proteins, primer etc. to be covalently labelled with the TA-label. The pre-activated TA-label is designated for the coupling with the target molecules through the reaction with primary amino groups which allows also double labelling strategies ideal for lateral flow test development projects.
“With the BioTeZ TAPAS Labeling Kit we react on the high demand on surface functionalization technologies for the diagnostic field. With our new TAPAS Labeling Kit we support lateral flow test manufacturing and contribute to a more convince to develop a new point of care technology” explains Dr. Janko Brand (Senior Scientist at BioTeZ).
The BioTeZ TAPAS Labeling Kit supports a large number of applications including immobilization, enrichment or detection of antibodies, other proteins and nucleic acids. It can be flexibly combined with various target molecules and detection systems such as fluorescent, luminescent, gold or carbon black (3).
Many commercial immunoassays employ the high affinity interaction between biotin and streptavidin as part of the analyte capture mechanism. As such, these immunoassays are subject to an interference if biotin is part of the diet (4). The new TAPAS Label is biotin free and will reduce the background and thereby increase the sensitivity for many applications.
“We are glad to demonstrate the ability of the TAPAS Labeling Kit for this important application in the coating and surface functionalization. Currently, we are in dialog with all major distributors and prepare a worldwide roll out of the BioTeZ TAPAS Labeling Kit” says Uwe Ahnert (CEO BioTeZ).
The advantages of the BioTeZ TAPAS Labeling Kit compared to conventional functionalization reagents are the improved signal noise ratio compared to streptavidin and poly-streptavidin. The high affinity binding between TA-Tag and PAS-Capture antibody leads to rapid binding with low cross-reactivity. Since the TA-PAS label is artificial and not present in nature non-specific binding were detected.
Product specifications: The BioTeZ TAPAS Labeling Kit includes the PAS capture antibody, all necessary reagents to label the target molecule with the TA-labels and clean-up columns for purifying the coupling product. For double labelling TA-label and gold nanoparticles it is recommended to do TA-labelling procedure first.
About BioTeZ: BioTeZ is a 1992 founded Biotech Company, developing and producing ELISA kits, protease activity kits, antibodies, diagnostics and oligonucleotides. BioTeZ has longstanding experience in the development of biochemical test procedures for research institutes, hospitals and laboratories worldwide. BioTeZ holds all relevant patents on the poly-streptavidin and TAPAS label production and distribution.
References
1. Zhang M, Biswas S, Deng W, Yu H. The Crystal Structure of Monovalent Streptavidin. Sci Rep 2016;6:35915.
2. Wang X, Du M, Mao G, Zheng J, Chen J, Ji X, et al. The synthesis of a smart streptavidin-functionalized poly(N-isopropylacrylamide) composite and its application in the separation and detection of virus nucleic acid. Talanta 2018;181:73-9.
3. Bové H, Steuwe C, Fron E, Slenders E, D’Haen J, Fujita Y, et al. Biocompatible Label-Free Detection of Carbon Black Particles by Femtosecond Pulsed Laser Microscopy. Nano Lett 2016;16:3173-8.
4. Gifford JL, de Koning L, Sadrzadeh SMH. Strategies for mitigating risk posed by biotin interference on clinical immunoassays. Clin Biochem 2018;
The strong interaction between streptavidin and biotin is widely utilized in biotechnological applications (1). The new TAPAS label was developed to extend the portfolio for assay manufactured and will be valuable for its applications across a wide range of biotechnological areas.
To meet the big demand BioTeZ introduced in 2008 the poly-streptavidin a crosslinked streptavidin polymer with increased biotin binding capacity widely used for lateral flow test applications (2). Now is 2018 BioTeZ released a novel surface functionalization platform the TAPAS Labeling Kit. This kit or labeling service allows an effective labeling a target molecules and detection with specific antibody.
The BioTeZ TAPAS Labeling Kit was developed to extend the portfolio for assay manufactured and will be valuable for its applications across a wide range of areas.The BioTeZ TAPAS Labeling Kit is a two component protein-ligand interaction system. One component is the small 1000 Dalton synthetic label called “TA-label”.
The second component is the “PAS-Antibody” which binds the “TA-label” with high specificity and efficiency. The BioTeZ TAPAS Labeling Kit or labelling service allows antibodies, proteins, primer etc. to be covalently labelled with the TA-label. The pre-activated TA-label is designated for the coupling with the target molecules through the reaction with primary amino groups which allows also double labelling strategies ideal for lateral flow test development projects.
“With the BioTeZ TAPAS Labeling Kit we react on the high demand on surface functionalization technologies for the diagnostic field. With our new TAPAS Labeling Kit we support lateral flow test manufacturing and contribute to a more convince to develop a new point of care technology” explains Dr. Janko Brand (Senior Scientist at BioTeZ).
The BioTeZ TAPAS Labeling Kit supports a large number of applications including immobilization, enrichment or detection of antibodies, other proteins and nucleic acids. It can be flexibly combined with various target molecules and detection systems such as fluorescent, luminescent, gold or carbon black (3).
Many commercial immunoassays employ the high affinity interaction between biotin and streptavidin as part of the analyte capture mechanism. As such, these immunoassays are subject to an interference if biotin is part of the diet (4). The new TAPAS Label is biotin free and will reduce the background and thereby increase the sensitivity for many applications.
“We are glad to demonstrate the ability of the TAPAS Labeling Kit for this important application in the coating and surface functionalization. Currently, we are in dialog with all major distributors and prepare a worldwide roll out of the BioTeZ TAPAS Labeling Kit” says Uwe Ahnert (CEO BioTeZ).
The advantages of the BioTeZ TAPAS Labeling Kit compared to conventional functionalization reagents are the improved signal noise ratio compared to streptavidin and poly-streptavidin. The high affinity binding between TA-Tag and PAS-Capture antibody leads to rapid binding with low cross-reactivity. Since the TA-PAS label is artificial and not present in nature non-specific binding were detected.
Product specifications: The BioTeZ TAPAS Labeling Kit includes the PAS capture antibody, all necessary reagents to label the target molecule with the TA-labels and clean-up columns for purifying the coupling product. For double labelling TA-label and gold nanoparticles it is recommended to do TA-labelling procedure first.
About BioTeZ: BioTeZ is a 1992 founded Biotech Company, developing and producing ELISA kits, protease activity kits, antibodies, diagnostics and oligonucleotides. BioTeZ has longstanding experience in the development of biochemical test procedures for research institutes, hospitals and laboratories worldwide. BioTeZ holds all relevant patents on the poly-streptavidin and TAPAS label production and distribution.
References
1. Zhang M, Biswas S, Deng W, Yu H. The Crystal Structure of Monovalent Streptavidin. Sci Rep 2016;6:35915.
2. Wang X, Du M, Mao G, Zheng J, Chen J, Ji X, et al. The synthesis of a smart streptavidin-functionalized poly(N-isopropylacrylamide) composite and its application in the separation and detection of virus nucleic acid. Talanta 2018;181:73-9.
3. Bové H, Steuwe C, Fron E, Slenders E, D’Haen J, Fujita Y, et al. Biocompatible Label-Free Detection of Carbon Black Particles by Femtosecond Pulsed Laser Microscopy. Nano Lett 2016;16:3173-8.
4. Gifford JL, de Koning L, Sadrzadeh SMH. Strategies for mitigating risk posed by biotin interference on clinical immunoassays. Clin Biochem 2018;
all-day
Tour De BoTeZ
We are happy to give you "Tour De BoTeZ" and walk you through the product & services we offer!
We provide You with the best biotechnology products and services that ensure high quality and innovation.
Founded in 1992 and ISO 9001:2015 certified, BioTeZ supports the research in many areas, both on the Biotechnology Campus Berlin-Buch, Germany, as well as in facilities around the world. For 25 years, we are continuously motivated for new challenges to meet Your needs. Since 2019 Steffens-Biotec, Ebringen, ISO 13485 certified is a sister company of BioTeZ specialized in manufacturing CE in-vitro diagnostics. We are specialized in immunochemistry and the development and production of high-quality components for ELISA, Immuno Assays & Protease Activity Kits, Immuno Affinity Chromatography and Lateral Flow Tests.
We provide You with the best biotechnology products and services that ensure high quality and innovation.
Founded in 1992 and ISO 9001:2015 certified, BioTeZ supports the research in many areas, both on the Biotechnology Campus Berlin-Buch, Germany, as well as in facilities around the world. For 25 years, we are continuously motivated for new challenges to meet Your needs. Since 2019 Steffens-Biotec, Ebringen, ISO 13485 certified is a sister company of BioTeZ specialized in manufacturing CE in-vitro diagnostics. We are specialized in immunochemistry and the development and production of high-quality components for ELISA, Immuno Assays & Protease Activity Kits, Immuno Affinity Chromatography and Lateral Flow Tests.
08:00 - 15:00
Demonstration of the In Vitro diagnostic ELISA of autoimmune & infectious diseases
Human-diagnostic ELISAs
The ELISA tests listed below consist of 29 kits for In Vitro diagnosis of autoimmune diseases and 2 kits for the diagnosis of a Helicobacter pylori infection.
These ELISA kits are CE marked and come in the standard format of 96 analyses. They all use identical general reagents, namely sample buffer, wash buffer, substrate and stop solution. Hence, one and the same sample dilution may be used for multiple assays; e.g. for simultaneous determination of Cardiolipin IgG and IgM.
All solutions are ready to use (exception: 10-fold wash buffer concentrate) and color-coded. The conjugate color (red, yellow or green) indicates the Ig-class detected, IgG, IgA or IgM, respectively. The color intensity of the blue calibrators reflects their corresponding concentration. The negative control is green, the positive red.
The solid phase features break apart microwells for economic use of the kit. It is packed in a foil bag with zip closure, useful for partial consumption of the solid phase. The frame of the microwell plate displays the parameter name.
The sample is serum or plasma; 10 µL are required throughout. It is diluted 1:100 in the orange coloured sample buffer. 100 µL per microwell is the fixed volume of sample, conjugate, substrate and stop solution.
The tests share one common protocol: 3 x 30 minutes incubation at room temperature and without shaking. Wash steps: unvariably 4 x 350 µL per microwell.
The tests have been validated on the Dynex DS2 system and are accessible to other automated devices. The liquids come in polyethylene bottles with enough volume for automation. The detection system is HRP/TMB, read at 450nm.
The compact outer box is labelled on two adjacent sides for easy test kit identification in fridge stacks. Each product comes with printed Directions for Use (also downloadable), including a Summary Flow Chart on the back, and a kit lot-specific Certificate of Analysis. Most of the tests offer the option of qualitative or quantitative evaluation.
The excellent quality of these products is reflected by a very low number of complaints over the years. Since more than a decade, we regularly participate in several international ring trials (UK: Neqas, US: CAP, Germany: Instand and Referenzinstitut für Bioanalytik) with virtually no deviation of our results from the respective intended response.
The ELISA tests listed below consist of 29 kits for In Vitro diagnosis of autoimmune diseases and 2 kits for the diagnosis of a Helicobacter pylori infection.
These ELISA kits are CE marked and come in the standard format of 96 analyses. They all use identical general reagents, namely sample buffer, wash buffer, substrate and stop solution. Hence, one and the same sample dilution may be used for multiple assays; e.g. for simultaneous determination of Cardiolipin IgG and IgM.
All solutions are ready to use (exception: 10-fold wash buffer concentrate) and color-coded. The conjugate color (red, yellow or green) indicates the Ig-class detected, IgG, IgA or IgM, respectively. The color intensity of the blue calibrators reflects their corresponding concentration. The negative control is green, the positive red.
The solid phase features break apart microwells for economic use of the kit. It is packed in a foil bag with zip closure, useful for partial consumption of the solid phase. The frame of the microwell plate displays the parameter name.
The sample is serum or plasma; 10 µL are required throughout. It is diluted 1:100 in the orange coloured sample buffer. 100 µL per microwell is the fixed volume of sample, conjugate, substrate and stop solution.
The tests share one common protocol: 3 x 30 minutes incubation at room temperature and without shaking. Wash steps: unvariably 4 x 350 µL per microwell.
The tests have been validated on the Dynex DS2 system and are accessible to other automated devices. The liquids come in polyethylene bottles with enough volume for automation. The detection system is HRP/TMB, read at 450nm.
The compact outer box is labelled on two adjacent sides for easy test kit identification in fridge stacks. Each product comes with printed Directions for Use (also downloadable), including a Summary Flow Chart on the back, and a kit lot-specific Certificate of Analysis. Most of the tests offer the option of qualitative or quantitative evaluation.
The excellent quality of these products is reflected by a very low number of complaints over the years. Since more than a decade, we regularly participate in several international ring trials (UK: Neqas, US: CAP, Germany: Instand and Referenzinstitut für Bioanalytik) with virtually no deviation of our results from the respective intended response.
19.11.2019
Topic
all-day
Demonstration of the TAPAS Lable
BioTeZ established a novel surface functionalization kit -the TAPAS system.
The strong interaction between streptavidin and biotin is widely utilized in biotechnological applications (1). The new TAPAS label was developed to extend the portfolio for assay manufactured and will be valuable for its applications across a wide range of biotechnological areas.
To meet the big demand BioTeZ introduced in 2008 the poly-streptavidin a crosslinked streptavidin polymer with increased biotin binding capacity widely used for lateral flow test applications (2). Now is 2018 BioTeZ released a novel surface functionalization platform the TAPAS Labeling Kit. This kit or labeling service allows an effective labeling a target molecules and detection with specific antibody.
The BioTeZ TAPAS Labeling Kit was developed to extend the portfolio for assay manufactured and will be valuable for its applications across a wide range of areas.The BioTeZ TAPAS Labeling Kit is a two component protein-ligand interaction system. One component is the small 1000 Dalton synthetic label called “TA-label”.
The second component is the “PAS-Antibody” which binds the “TA-label” with high specificity and efficiency. The BioTeZ TAPAS Labeling Kit or labelling service allows antibodies, proteins, primer etc. to be covalently labelled with the TA-label. The pre-activated TA-label is designated for the coupling with the target molecules through the reaction with primary amino groups which allows also double labelling strategies ideal for lateral flow test development projects.
“With the BioTeZ TAPAS Labeling Kit we react on the high demand on surface functionalization technologies for the diagnostic field. With our new TAPAS Labeling Kit we support lateral flow test manufacturing and contribute to a more convince to develop a new point of care technology” explains Dr. Janko Brand (Senior Scientist at BioTeZ).
The BioTeZ TAPAS Labeling Kit supports a large number of applications including immobilization, enrichment or detection of antibodies, other proteins and nucleic acids. It can be flexibly combined with various target molecules and detection systems such as fluorescent, luminescent, gold or carbon black (3).
Many commercial immunoassays employ the high affinity interaction between biotin and streptavidin as part of the analyte capture mechanism. As such, these immunoassays are subject to an interference if biotin is part of the diet (4). The new TAPAS Label is biotin free and will reduce the background and thereby increase the sensitivity for many applications.
“We are glad to demonstrate the ability of the TAPAS Labeling Kit for this important application in the coating and surface functionalization. Currently, we are in dialog with all major distributors and prepare a worldwide roll out of the BioTeZ TAPAS Labeling Kit” says Uwe Ahnert (CEO BioTeZ).
The advantages of the BioTeZ TAPAS Labeling Kit compared to conventional functionalization reagents are the improved signal noise ratio compared to streptavidin and poly-streptavidin. The high affinity binding between TA-Tag and PAS-Capture antibody leads to rapid binding with low cross-reactivity. Since the TA-PAS label is artificial and not present in nature non-specific binding were detected.
Product specifications: The BioTeZ TAPAS Labeling Kit includes the PAS capture antibody, all necessary reagents to label the target molecule with the TA-labels and clean-up columns for purifying the coupling product. For double labelling TA-label and gold nanoparticles it is recommended to do TA-labelling procedure first.
About BioTeZ: BioTeZ is a 1992 founded Biotech Company, developing and producing ELISA kits, protease activity kits, antibodies, diagnostics and oligonucleotides. BioTeZ has longstanding experience in the development of biochemical test procedures for research institutes, hospitals and laboratories worldwide. BioTeZ holds all relevant patents on the poly-streptavidin and TAPAS label production and distribution.
References
1. Zhang M, Biswas S, Deng W, Yu H. The Crystal Structure of Monovalent Streptavidin. Sci Rep 2016;6:35915.
2. Wang X, Du M, Mao G, Zheng J, Chen J, Ji X, et al. The synthesis of a smart streptavidin-functionalized poly(N-isopropylacrylamide) composite and its application in the separation and detection of virus nucleic acid. Talanta 2018;181:73-9.
3. Bové H, Steuwe C, Fron E, Slenders E, D’Haen J, Fujita Y, et al. Biocompatible Label-Free Detection of Carbon Black Particles by Femtosecond Pulsed Laser Microscopy. Nano Lett 2016;16:3173-8.
4. Gifford JL, de Koning L, Sadrzadeh SMH. Strategies for mitigating risk posed by biotin interference on clinical immunoassays. Clin Biochem 2018;
The strong interaction between streptavidin and biotin is widely utilized in biotechnological applications (1). The new TAPAS label was developed to extend the portfolio for assay manufactured and will be valuable for its applications across a wide range of biotechnological areas.
To meet the big demand BioTeZ introduced in 2008 the poly-streptavidin a crosslinked streptavidin polymer with increased biotin binding capacity widely used for lateral flow test applications (2). Now is 2018 BioTeZ released a novel surface functionalization platform the TAPAS Labeling Kit. This kit or labeling service allows an effective labeling a target molecules and detection with specific antibody.
The BioTeZ TAPAS Labeling Kit was developed to extend the portfolio for assay manufactured and will be valuable for its applications across a wide range of areas.The BioTeZ TAPAS Labeling Kit is a two component protein-ligand interaction system. One component is the small 1000 Dalton synthetic label called “TA-label”.
The second component is the “PAS-Antibody” which binds the “TA-label” with high specificity and efficiency. The BioTeZ TAPAS Labeling Kit or labelling service allows antibodies, proteins, primer etc. to be covalently labelled with the TA-label. The pre-activated TA-label is designated for the coupling with the target molecules through the reaction with primary amino groups which allows also double labelling strategies ideal for lateral flow test development projects.
“With the BioTeZ TAPAS Labeling Kit we react on the high demand on surface functionalization technologies for the diagnostic field. With our new TAPAS Labeling Kit we support lateral flow test manufacturing and contribute to a more convince to develop a new point of care technology” explains Dr. Janko Brand (Senior Scientist at BioTeZ).
The BioTeZ TAPAS Labeling Kit supports a large number of applications including immobilization, enrichment or detection of antibodies, other proteins and nucleic acids. It can be flexibly combined with various target molecules and detection systems such as fluorescent, luminescent, gold or carbon black (3).
Many commercial immunoassays employ the high affinity interaction between biotin and streptavidin as part of the analyte capture mechanism. As such, these immunoassays are subject to an interference if biotin is part of the diet (4). The new TAPAS Label is biotin free and will reduce the background and thereby increase the sensitivity for many applications.
“We are glad to demonstrate the ability of the TAPAS Labeling Kit for this important application in the coating and surface functionalization. Currently, we are in dialog with all major distributors and prepare a worldwide roll out of the BioTeZ TAPAS Labeling Kit” says Uwe Ahnert (CEO BioTeZ).
The advantages of the BioTeZ TAPAS Labeling Kit compared to conventional functionalization reagents are the improved signal noise ratio compared to streptavidin and poly-streptavidin. The high affinity binding between TA-Tag and PAS-Capture antibody leads to rapid binding with low cross-reactivity. Since the TA-PAS label is artificial and not present in nature non-specific binding were detected.
Product specifications: The BioTeZ TAPAS Labeling Kit includes the PAS capture antibody, all necessary reagents to label the target molecule with the TA-labels and clean-up columns for purifying the coupling product. For double labelling TA-label and gold nanoparticles it is recommended to do TA-labelling procedure first.
About BioTeZ: BioTeZ is a 1992 founded Biotech Company, developing and producing ELISA kits, protease activity kits, antibodies, diagnostics and oligonucleotides. BioTeZ has longstanding experience in the development of biochemical test procedures for research institutes, hospitals and laboratories worldwide. BioTeZ holds all relevant patents on the poly-streptavidin and TAPAS label production and distribution.
References
1. Zhang M, Biswas S, Deng W, Yu H. The Crystal Structure of Monovalent Streptavidin. Sci Rep 2016;6:35915.
2. Wang X, Du M, Mao G, Zheng J, Chen J, Ji X, et al. The synthesis of a smart streptavidin-functionalized poly(N-isopropylacrylamide) composite and its application in the separation and detection of virus nucleic acid. Talanta 2018;181:73-9.
3. Bové H, Steuwe C, Fron E, Slenders E, D’Haen J, Fujita Y, et al. Biocompatible Label-Free Detection of Carbon Black Particles by Femtosecond Pulsed Laser Microscopy. Nano Lett 2016;16:3173-8.
4. Gifford JL, de Koning L, Sadrzadeh SMH. Strategies for mitigating risk posed by biotin interference on clinical immunoassays. Clin Biochem 2018;
all-day
Tour De BoTeZ
We are happy to give you "Tour De BoTeZ" and walk you through the product & services we offer!
We provide You with the best biotechnology products and services that ensure high quality and innovation.
Founded in 1992 and ISO 9001:2015 certified, BioTeZ supports the research in many areas, both on the Biotechnology Campus Berlin-Buch, Germany, as well as in facilities around the world. For 25 years, we are continuously motivated for new challenges to meet Your needs. Since 2019 Steffens-Biotec, Ebringen, ISO 13485 certified is a sister company of BioTeZ specialized in manufacturing CE in-vitro diagnostics. We are specialized in immunochemistry and the development and production of high-quality components for ELISA, Immuno Assays & Protease Activity Kits, Immuno Affinity Chromatography and Lateral Flow Tests.
We provide You with the best biotechnology products and services that ensure high quality and innovation.
Founded in 1992 and ISO 9001:2015 certified, BioTeZ supports the research in many areas, both on the Biotechnology Campus Berlin-Buch, Germany, as well as in facilities around the world. For 25 years, we are continuously motivated for new challenges to meet Your needs. Since 2019 Steffens-Biotec, Ebringen, ISO 13485 certified is a sister company of BioTeZ specialized in manufacturing CE in-vitro diagnostics. We are specialized in immunochemistry and the development and production of high-quality components for ELISA, Immuno Assays & Protease Activity Kits, Immuno Affinity Chromatography and Lateral Flow Tests.
08:00 - 15:00
Demonstration of the In Vitro diagnostic ELISA of autoimmune & infectious diseases
Human-diagnostic ELISAs
The ELISA tests listed below consist of 29 kits for In Vitro diagnosis of autoimmune diseases and 2 kits for the diagnosis of a Helicobacter pylori infection.
These ELISA kits are CE marked and come in the standard format of 96 analyses. They all use identical general reagents, namely sample buffer, wash buffer, substrate and stop solution. Hence, one and the same sample dilution may be used for multiple assays; e.g. for simultaneous determination of Cardiolipin IgG and IgM.
All solutions are ready to use (exception: 10-fold wash buffer concentrate) and color-coded. The conjugate color (red, yellow or green) indicates the Ig-class detected, IgG, IgA or IgM, respectively. The color intensity of the blue calibrators reflects their corresponding concentration. The negative control is green, the positive red.
The solid phase features break apart microwells for economic use of the kit. It is packed in a foil bag with zip closure, useful for partial consumption of the solid phase. The frame of the microwell plate displays the parameter name.
The sample is serum or plasma; 10 µL are required throughout. It is diluted 1:100 in the orange coloured sample buffer. 100 µL per microwell is the fixed volume of sample, conjugate, substrate and stop solution.
The tests share one common protocol: 3 x 30 minutes incubation at room temperature and without shaking. Wash steps: unvariably 4 x 350 µL per microwell.
The tests have been validated on the Dynex DS2 system and are accessible to other automated devices. The liquids come in polyethylene bottles with enough volume for automation. The detection system is HRP/TMB, read at 450nm.
The compact outer box is labelled on two adjacent sides for easy test kit identification in fridge stacks. Each product comes with printed Directions for Use (also downloadable), including a Summary Flow Chart on the back, and a kit lot-specific Certificate of Analysis. Most of the tests offer the option of qualitative or quantitative evaluation.
The excellent quality of these products is reflected by a very low number of complaints over the years. Since more than a decade, we regularly participate in several international ring trials (UK: Neqas, US: CAP, Germany: Instand and Referenzinstitut für Bioanalytik) with virtually no deviation of our results from the respective intended response.
The ELISA tests listed below consist of 29 kits for In Vitro diagnosis of autoimmune diseases and 2 kits for the diagnosis of a Helicobacter pylori infection.
These ELISA kits are CE marked and come in the standard format of 96 analyses. They all use identical general reagents, namely sample buffer, wash buffer, substrate and stop solution. Hence, one and the same sample dilution may be used for multiple assays; e.g. for simultaneous determination of Cardiolipin IgG and IgM.
All solutions are ready to use (exception: 10-fold wash buffer concentrate) and color-coded. The conjugate color (red, yellow or green) indicates the Ig-class detected, IgG, IgA or IgM, respectively. The color intensity of the blue calibrators reflects their corresponding concentration. The negative control is green, the positive red.
The solid phase features break apart microwells for economic use of the kit. It is packed in a foil bag with zip closure, useful for partial consumption of the solid phase. The frame of the microwell plate displays the parameter name.
The sample is serum or plasma; 10 µL are required throughout. It is diluted 1:100 in the orange coloured sample buffer. 100 µL per microwell is the fixed volume of sample, conjugate, substrate and stop solution.
The tests share one common protocol: 3 x 30 minutes incubation at room temperature and without shaking. Wash steps: unvariably 4 x 350 µL per microwell.
The tests have been validated on the Dynex DS2 system and are accessible to other automated devices. The liquids come in polyethylene bottles with enough volume for automation. The detection system is HRP/TMB, read at 450nm.
The compact outer box is labelled on two adjacent sides for easy test kit identification in fridge stacks. Each product comes with printed Directions for Use (also downloadable), including a Summary Flow Chart on the back, and a kit lot-specific Certificate of Analysis. Most of the tests offer the option of qualitative or quantitative evaluation.
The excellent quality of these products is reflected by a very low number of complaints over the years. Since more than a decade, we regularly participate in several international ring trials (UK: Neqas, US: CAP, Germany: Instand and Referenzinstitut für Bioanalytik) with virtually no deviation of our results from the respective intended response.
20.11.2019
Topic
all-day
Demonstration of the TAPAS Lable
BioTeZ established a novel surface functionalization kit -the TAPAS system.
The strong interaction between streptavidin and biotin is widely utilized in biotechnological applications (1). The new TAPAS label was developed to extend the portfolio for assay manufactured and will be valuable for its applications across a wide range of biotechnological areas.
To meet the big demand BioTeZ introduced in 2008 the poly-streptavidin a crosslinked streptavidin polymer with increased biotin binding capacity widely used for lateral flow test applications (2). Now is 2018 BioTeZ released a novel surface functionalization platform the TAPAS Labeling Kit. This kit or labeling service allows an effective labeling a target molecules and detection with specific antibody.
The BioTeZ TAPAS Labeling Kit was developed to extend the portfolio for assay manufactured and will be valuable for its applications across a wide range of areas.The BioTeZ TAPAS Labeling Kit is a two component protein-ligand interaction system. One component is the small 1000 Dalton synthetic label called “TA-label”.
The second component is the “PAS-Antibody” which binds the “TA-label” with high specificity and efficiency. The BioTeZ TAPAS Labeling Kit or labelling service allows antibodies, proteins, primer etc. to be covalently labelled with the TA-label. The pre-activated TA-label is designated for the coupling with the target molecules through the reaction with primary amino groups which allows also double labelling strategies ideal for lateral flow test development projects.
“With the BioTeZ TAPAS Labeling Kit we react on the high demand on surface functionalization technologies for the diagnostic field. With our new TAPAS Labeling Kit we support lateral flow test manufacturing and contribute to a more convince to develop a new point of care technology” explains Dr. Janko Brand (Senior Scientist at BioTeZ).
The BioTeZ TAPAS Labeling Kit supports a large number of applications including immobilization, enrichment or detection of antibodies, other proteins and nucleic acids. It can be flexibly combined with various target molecules and detection systems such as fluorescent, luminescent, gold or carbon black (3).
Many commercial immunoassays employ the high affinity interaction between biotin and streptavidin as part of the analyte capture mechanism. As such, these immunoassays are subject to an interference if biotin is part of the diet (4). The new TAPAS Label is biotin free and will reduce the background and thereby increase the sensitivity for many applications.
“We are glad to demonstrate the ability of the TAPAS Labeling Kit for this important application in the coating and surface functionalization. Currently, we are in dialog with all major distributors and prepare a worldwide roll out of the BioTeZ TAPAS Labeling Kit” says Uwe Ahnert (CEO BioTeZ).
The advantages of the BioTeZ TAPAS Labeling Kit compared to conventional functionalization reagents are the improved signal noise ratio compared to streptavidin and poly-streptavidin. The high affinity binding between TA-Tag and PAS-Capture antibody leads to rapid binding with low cross-reactivity. Since the TA-PAS label is artificial and not present in nature non-specific binding were detected.
Product specifications: The BioTeZ TAPAS Labeling Kit includes the PAS capture antibody, all necessary reagents to label the target molecule with the TA-labels and clean-up columns for purifying the coupling product. For double labelling TA-label and gold nanoparticles it is recommended to do TA-labelling procedure first.
About BioTeZ: BioTeZ is a 1992 founded Biotech Company, developing and producing ELISA kits, protease activity kits, antibodies, diagnostics and oligonucleotides. BioTeZ has longstanding experience in the development of biochemical test procedures for research institutes, hospitals and laboratories worldwide. BioTeZ holds all relevant patents on the poly-streptavidin and TAPAS label production and distribution.
References
1. Zhang M, Biswas S, Deng W, Yu H. The Crystal Structure of Monovalent Streptavidin. Sci Rep 2016;6:35915.
2. Wang X, Du M, Mao G, Zheng J, Chen J, Ji X, et al. The synthesis of a smart streptavidin-functionalized poly(N-isopropylacrylamide) composite and its application in the separation and detection of virus nucleic acid. Talanta 2018;181:73-9.
3. Bové H, Steuwe C, Fron E, Slenders E, D’Haen J, Fujita Y, et al. Biocompatible Label-Free Detection of Carbon Black Particles by Femtosecond Pulsed Laser Microscopy. Nano Lett 2016;16:3173-8.
4. Gifford JL, de Koning L, Sadrzadeh SMH. Strategies for mitigating risk posed by biotin interference on clinical immunoassays. Clin Biochem 2018;
The strong interaction between streptavidin and biotin is widely utilized in biotechnological applications (1). The new TAPAS label was developed to extend the portfolio for assay manufactured and will be valuable for its applications across a wide range of biotechnological areas.
To meet the big demand BioTeZ introduced in 2008 the poly-streptavidin a crosslinked streptavidin polymer with increased biotin binding capacity widely used for lateral flow test applications (2). Now is 2018 BioTeZ released a novel surface functionalization platform the TAPAS Labeling Kit. This kit or labeling service allows an effective labeling a target molecules and detection with specific antibody.
The BioTeZ TAPAS Labeling Kit was developed to extend the portfolio for assay manufactured and will be valuable for its applications across a wide range of areas.The BioTeZ TAPAS Labeling Kit is a two component protein-ligand interaction system. One component is the small 1000 Dalton synthetic label called “TA-label”.
The second component is the “PAS-Antibody” which binds the “TA-label” with high specificity and efficiency. The BioTeZ TAPAS Labeling Kit or labelling service allows antibodies, proteins, primer etc. to be covalently labelled with the TA-label. The pre-activated TA-label is designated for the coupling with the target molecules through the reaction with primary amino groups which allows also double labelling strategies ideal for lateral flow test development projects.
“With the BioTeZ TAPAS Labeling Kit we react on the high demand on surface functionalization technologies for the diagnostic field. With our new TAPAS Labeling Kit we support lateral flow test manufacturing and contribute to a more convince to develop a new point of care technology” explains Dr. Janko Brand (Senior Scientist at BioTeZ).
The BioTeZ TAPAS Labeling Kit supports a large number of applications including immobilization, enrichment or detection of antibodies, other proteins and nucleic acids. It can be flexibly combined with various target molecules and detection systems such as fluorescent, luminescent, gold or carbon black (3).
Many commercial immunoassays employ the high affinity interaction between biotin and streptavidin as part of the analyte capture mechanism. As such, these immunoassays are subject to an interference if biotin is part of the diet (4). The new TAPAS Label is biotin free and will reduce the background and thereby increase the sensitivity for many applications.
“We are glad to demonstrate the ability of the TAPAS Labeling Kit for this important application in the coating and surface functionalization. Currently, we are in dialog with all major distributors and prepare a worldwide roll out of the BioTeZ TAPAS Labeling Kit” says Uwe Ahnert (CEO BioTeZ).
The advantages of the BioTeZ TAPAS Labeling Kit compared to conventional functionalization reagents are the improved signal noise ratio compared to streptavidin and poly-streptavidin. The high affinity binding between TA-Tag and PAS-Capture antibody leads to rapid binding with low cross-reactivity. Since the TA-PAS label is artificial and not present in nature non-specific binding were detected.
Product specifications: The BioTeZ TAPAS Labeling Kit includes the PAS capture antibody, all necessary reagents to label the target molecule with the TA-labels and clean-up columns for purifying the coupling product. For double labelling TA-label and gold nanoparticles it is recommended to do TA-labelling procedure first.
About BioTeZ: BioTeZ is a 1992 founded Biotech Company, developing and producing ELISA kits, protease activity kits, antibodies, diagnostics and oligonucleotides. BioTeZ has longstanding experience in the development of biochemical test procedures for research institutes, hospitals and laboratories worldwide. BioTeZ holds all relevant patents on the poly-streptavidin and TAPAS label production and distribution.
References
1. Zhang M, Biswas S, Deng W, Yu H. The Crystal Structure of Monovalent Streptavidin. Sci Rep 2016;6:35915.
2. Wang X, Du M, Mao G, Zheng J, Chen J, Ji X, et al. The synthesis of a smart streptavidin-functionalized poly(N-isopropylacrylamide) composite and its application in the separation and detection of virus nucleic acid. Talanta 2018;181:73-9.
3. Bové H, Steuwe C, Fron E, Slenders E, D’Haen J, Fujita Y, et al. Biocompatible Label-Free Detection of Carbon Black Particles by Femtosecond Pulsed Laser Microscopy. Nano Lett 2016;16:3173-8.
4. Gifford JL, de Koning L, Sadrzadeh SMH. Strategies for mitigating risk posed by biotin interference on clinical immunoassays. Clin Biochem 2018;
all-day
Tour De BoTeZ
We are happy to give you "Tour De BoTeZ" and walk you through the product & services we offer!
We provide You with the best biotechnology products and services that ensure high quality and innovation.
Founded in 1992 and ISO 9001:2015 certified, BioTeZ supports the research in many areas, both on the Biotechnology Campus Berlin-Buch, Germany, as well as in facilities around the world. For 25 years, we are continuously motivated for new challenges to meet Your needs. Since 2019 Steffens-Biotec, Ebringen, ISO 13485 certified is a sister company of BioTeZ specialized in manufacturing CE in-vitro diagnostics. We are specialized in immunochemistry and the development and production of high-quality components for ELISA, Immuno Assays & Protease Activity Kits, Immuno Affinity Chromatography and Lateral Flow Tests.
We provide You with the best biotechnology products and services that ensure high quality and innovation.
Founded in 1992 and ISO 9001:2015 certified, BioTeZ supports the research in many areas, both on the Biotechnology Campus Berlin-Buch, Germany, as well as in facilities around the world. For 25 years, we are continuously motivated for new challenges to meet Your needs. Since 2019 Steffens-Biotec, Ebringen, ISO 13485 certified is a sister company of BioTeZ specialized in manufacturing CE in-vitro diagnostics. We are specialized in immunochemistry and the development and production of high-quality components for ELISA, Immuno Assays & Protease Activity Kits, Immuno Affinity Chromatography and Lateral Flow Tests.
08:00 - 15:00
Demonstration of the In Vitro diagnostic ELISA of autoimmune & infectious diseases
Human-diagnostic ELISAs
The ELISA tests listed below consist of 29 kits for In Vitro diagnosis of autoimmune diseases and 2 kits for the diagnosis of a Helicobacter pylori infection.
These ELISA kits are CE marked and come in the standard format of 96 analyses. They all use identical general reagents, namely sample buffer, wash buffer, substrate and stop solution. Hence, one and the same sample dilution may be used for multiple assays; e.g. for simultaneous determination of Cardiolipin IgG and IgM.
All solutions are ready to use (exception: 10-fold wash buffer concentrate) and color-coded. The conjugate color (red, yellow or green) indicates the Ig-class detected, IgG, IgA or IgM, respectively. The color intensity of the blue calibrators reflects their corresponding concentration. The negative control is green, the positive red.
The solid phase features break apart microwells for economic use of the kit. It is packed in a foil bag with zip closure, useful for partial consumption of the solid phase. The frame of the microwell plate displays the parameter name.
The sample is serum or plasma; 10 µL are required throughout. It is diluted 1:100 in the orange coloured sample buffer. 100 µL per microwell is the fixed volume of sample, conjugate, substrate and stop solution.
The tests share one common protocol: 3 x 30 minutes incubation at room temperature and without shaking. Wash steps: unvariably 4 x 350 µL per microwell.
The tests have been validated on the Dynex DS2 system and are accessible to other automated devices. The liquids come in polyethylene bottles with enough volume for automation. The detection system is HRP/TMB, read at 450nm.
The compact outer box is labelled on two adjacent sides for easy test kit identification in fridge stacks. Each product comes with printed Directions for Use (also downloadable), including a Summary Flow Chart on the back, and a kit lot-specific Certificate of Analysis. Most of the tests offer the option of qualitative or quantitative evaluation.
The excellent quality of these products is reflected by a very low number of complaints over the years. Since more than a decade, we regularly participate in several international ring trials (UK: Neqas, US: CAP, Germany: Instand and Referenzinstitut für Bioanalytik) with virtually no deviation of our results from the respective intended response.
The ELISA tests listed below consist of 29 kits for In Vitro diagnosis of autoimmune diseases and 2 kits for the diagnosis of a Helicobacter pylori infection.
These ELISA kits are CE marked and come in the standard format of 96 analyses. They all use identical general reagents, namely sample buffer, wash buffer, substrate and stop solution. Hence, one and the same sample dilution may be used for multiple assays; e.g. for simultaneous determination of Cardiolipin IgG and IgM.
All solutions are ready to use (exception: 10-fold wash buffer concentrate) and color-coded. The conjugate color (red, yellow or green) indicates the Ig-class detected, IgG, IgA or IgM, respectively. The color intensity of the blue calibrators reflects their corresponding concentration. The negative control is green, the positive red.
The solid phase features break apart microwells for economic use of the kit. It is packed in a foil bag with zip closure, useful for partial consumption of the solid phase. The frame of the microwell plate displays the parameter name.
The sample is serum or plasma; 10 µL are required throughout. It is diluted 1:100 in the orange coloured sample buffer. 100 µL per microwell is the fixed volume of sample, conjugate, substrate and stop solution.
The tests share one common protocol: 3 x 30 minutes incubation at room temperature and without shaking. Wash steps: unvariably 4 x 350 µL per microwell.
The tests have been validated on the Dynex DS2 system and are accessible to other automated devices. The liquids come in polyethylene bottles with enough volume for automation. The detection system is HRP/TMB, read at 450nm.
The compact outer box is labelled on two adjacent sides for easy test kit identification in fridge stacks. Each product comes with printed Directions for Use (also downloadable), including a Summary Flow Chart on the back, and a kit lot-specific Certificate of Analysis. Most of the tests offer the option of qualitative or quantitative evaluation.
The excellent quality of these products is reflected by a very low number of complaints over the years. Since more than a decade, we regularly participate in several international ring trials (UK: Neqas, US: CAP, Germany: Instand and Referenzinstitut für Bioanalytik) with virtually no deviation of our results from the respective intended response.
21.11.2019
Topic
all-day
Demonstration of the TAPAS Lable
BioTeZ established a novel surface functionalization kit -the TAPAS system.
The strong interaction between streptavidin and biotin is widely utilized in biotechnological applications (1). The new TAPAS label was developed to extend the portfolio for assay manufactured and will be valuable for its applications across a wide range of biotechnological areas.
To meet the big demand BioTeZ introduced in 2008 the poly-streptavidin a crosslinked streptavidin polymer with increased biotin binding capacity widely used for lateral flow test applications (2). Now is 2018 BioTeZ released a novel surface functionalization platform the TAPAS Labeling Kit. This kit or labeling service allows an effective labeling a target molecules and detection with specific antibody.
The BioTeZ TAPAS Labeling Kit was developed to extend the portfolio for assay manufactured and will be valuable for its applications across a wide range of areas.The BioTeZ TAPAS Labeling Kit is a two component protein-ligand interaction system. One component is the small 1000 Dalton synthetic label called “TA-label”.
The second component is the “PAS-Antibody” which binds the “TA-label” with high specificity and efficiency. The BioTeZ TAPAS Labeling Kit or labelling service allows antibodies, proteins, primer etc. to be covalently labelled with the TA-label. The pre-activated TA-label is designated for the coupling with the target molecules through the reaction with primary amino groups which allows also double labelling strategies ideal for lateral flow test development projects.
“With the BioTeZ TAPAS Labeling Kit we react on the high demand on surface functionalization technologies for the diagnostic field. With our new TAPAS Labeling Kit we support lateral flow test manufacturing and contribute to a more convince to develop a new point of care technology” explains Dr. Janko Brand (Senior Scientist at BioTeZ).
The BioTeZ TAPAS Labeling Kit supports a large number of applications including immobilization, enrichment or detection of antibodies, other proteins and nucleic acids. It can be flexibly combined with various target molecules and detection systems such as fluorescent, luminescent, gold or carbon black (3).
Many commercial immunoassays employ the high affinity interaction between biotin and streptavidin as part of the analyte capture mechanism. As such, these immunoassays are subject to an interference if biotin is part of the diet (4). The new TAPAS Label is biotin free and will reduce the background and thereby increase the sensitivity for many applications.
“We are glad to demonstrate the ability of the TAPAS Labeling Kit for this important application in the coating and surface functionalization. Currently, we are in dialog with all major distributors and prepare a worldwide roll out of the BioTeZ TAPAS Labeling Kit” says Uwe Ahnert (CEO BioTeZ).
The advantages of the BioTeZ TAPAS Labeling Kit compared to conventional functionalization reagents are the improved signal noise ratio compared to streptavidin and poly-streptavidin. The high affinity binding between TA-Tag and PAS-Capture antibody leads to rapid binding with low cross-reactivity. Since the TA-PAS label is artificial and not present in nature non-specific binding were detected.
Product specifications: The BioTeZ TAPAS Labeling Kit includes the PAS capture antibody, all necessary reagents to label the target molecule with the TA-labels and clean-up columns for purifying the coupling product. For double labelling TA-label and gold nanoparticles it is recommended to do TA-labelling procedure first.
About BioTeZ: BioTeZ is a 1992 founded Biotech Company, developing and producing ELISA kits, protease activity kits, antibodies, diagnostics and oligonucleotides. BioTeZ has longstanding experience in the development of biochemical test procedures for research institutes, hospitals and laboratories worldwide. BioTeZ holds all relevant patents on the poly-streptavidin and TAPAS label production and distribution.
References
1. Zhang M, Biswas S, Deng W, Yu H. The Crystal Structure of Monovalent Streptavidin. Sci Rep 2016;6:35915.
2. Wang X, Du M, Mao G, Zheng J, Chen J, Ji X, et al. The synthesis of a smart streptavidin-functionalized poly(N-isopropylacrylamide) composite and its application in the separation and detection of virus nucleic acid. Talanta 2018;181:73-9.
3. Bové H, Steuwe C, Fron E, Slenders E, D’Haen J, Fujita Y, et al. Biocompatible Label-Free Detection of Carbon Black Particles by Femtosecond Pulsed Laser Microscopy. Nano Lett 2016;16:3173-8.
4. Gifford JL, de Koning L, Sadrzadeh SMH. Strategies for mitigating risk posed by biotin interference on clinical immunoassays. Clin Biochem 2018;
The strong interaction between streptavidin and biotin is widely utilized in biotechnological applications (1). The new TAPAS label was developed to extend the portfolio for assay manufactured and will be valuable for its applications across a wide range of biotechnological areas.
To meet the big demand BioTeZ introduced in 2008 the poly-streptavidin a crosslinked streptavidin polymer with increased biotin binding capacity widely used for lateral flow test applications (2). Now is 2018 BioTeZ released a novel surface functionalization platform the TAPAS Labeling Kit. This kit or labeling service allows an effective labeling a target molecules and detection with specific antibody.
The BioTeZ TAPAS Labeling Kit was developed to extend the portfolio for assay manufactured and will be valuable for its applications across a wide range of areas.The BioTeZ TAPAS Labeling Kit is a two component protein-ligand interaction system. One component is the small 1000 Dalton synthetic label called “TA-label”.
The second component is the “PAS-Antibody” which binds the “TA-label” with high specificity and efficiency. The BioTeZ TAPAS Labeling Kit or labelling service allows antibodies, proteins, primer etc. to be covalently labelled with the TA-label. The pre-activated TA-label is designated for the coupling with the target molecules through the reaction with primary amino groups which allows also double labelling strategies ideal for lateral flow test development projects.
“With the BioTeZ TAPAS Labeling Kit we react on the high demand on surface functionalization technologies for the diagnostic field. With our new TAPAS Labeling Kit we support lateral flow test manufacturing and contribute to a more convince to develop a new point of care technology” explains Dr. Janko Brand (Senior Scientist at BioTeZ).
The BioTeZ TAPAS Labeling Kit supports a large number of applications including immobilization, enrichment or detection of antibodies, other proteins and nucleic acids. It can be flexibly combined with various target molecules and detection systems such as fluorescent, luminescent, gold or carbon black (3).
Many commercial immunoassays employ the high affinity interaction between biotin and streptavidin as part of the analyte capture mechanism. As such, these immunoassays are subject to an interference if biotin is part of the diet (4). The new TAPAS Label is biotin free and will reduce the background and thereby increase the sensitivity for many applications.
“We are glad to demonstrate the ability of the TAPAS Labeling Kit for this important application in the coating and surface functionalization. Currently, we are in dialog with all major distributors and prepare a worldwide roll out of the BioTeZ TAPAS Labeling Kit” says Uwe Ahnert (CEO BioTeZ).
The advantages of the BioTeZ TAPAS Labeling Kit compared to conventional functionalization reagents are the improved signal noise ratio compared to streptavidin and poly-streptavidin. The high affinity binding between TA-Tag and PAS-Capture antibody leads to rapid binding with low cross-reactivity. Since the TA-PAS label is artificial and not present in nature non-specific binding were detected.
Product specifications: The BioTeZ TAPAS Labeling Kit includes the PAS capture antibody, all necessary reagents to label the target molecule with the TA-labels and clean-up columns for purifying the coupling product. For double labelling TA-label and gold nanoparticles it is recommended to do TA-labelling procedure first.
About BioTeZ: BioTeZ is a 1992 founded Biotech Company, developing and producing ELISA kits, protease activity kits, antibodies, diagnostics and oligonucleotides. BioTeZ has longstanding experience in the development of biochemical test procedures for research institutes, hospitals and laboratories worldwide. BioTeZ holds all relevant patents on the poly-streptavidin and TAPAS label production and distribution.
References
1. Zhang M, Biswas S, Deng W, Yu H. The Crystal Structure of Monovalent Streptavidin. Sci Rep 2016;6:35915.
2. Wang X, Du M, Mao G, Zheng J, Chen J, Ji X, et al. The synthesis of a smart streptavidin-functionalized poly(N-isopropylacrylamide) composite and its application in the separation and detection of virus nucleic acid. Talanta 2018;181:73-9.
3. Bové H, Steuwe C, Fron E, Slenders E, D’Haen J, Fujita Y, et al. Biocompatible Label-Free Detection of Carbon Black Particles by Femtosecond Pulsed Laser Microscopy. Nano Lett 2016;16:3173-8.
4. Gifford JL, de Koning L, Sadrzadeh SMH. Strategies for mitigating risk posed by biotin interference on clinical immunoassays. Clin Biochem 2018;
all-day
Tour De BoTeZ
We are happy to give you "Tour De BoTeZ" and walk you through the product & services we offer!
We provide You with the best biotechnology products and services that ensure high quality and innovation.
Founded in 1992 and ISO 9001:2015 certified, BioTeZ supports the research in many areas, both on the Biotechnology Campus Berlin-Buch, Germany, as well as in facilities around the world. For 25 years, we are continuously motivated for new challenges to meet Your needs. Since 2019 Steffens-Biotec, Ebringen, ISO 13485 certified is a sister company of BioTeZ specialized in manufacturing CE in-vitro diagnostics. We are specialized in immunochemistry and the development and production of high-quality components for ELISA, Immuno Assays & Protease Activity Kits, Immuno Affinity Chromatography and Lateral Flow Tests.
We provide You with the best biotechnology products and services that ensure high quality and innovation.
Founded in 1992 and ISO 9001:2015 certified, BioTeZ supports the research in many areas, both on the Biotechnology Campus Berlin-Buch, Germany, as well as in facilities around the world. For 25 years, we are continuously motivated for new challenges to meet Your needs. Since 2019 Steffens-Biotec, Ebringen, ISO 13485 certified is a sister company of BioTeZ specialized in manufacturing CE in-vitro diagnostics. We are specialized in immunochemistry and the development and production of high-quality components for ELISA, Immuno Assays & Protease Activity Kits, Immuno Affinity Chromatography and Lateral Flow Tests.
08:00 - 15:00
Demonstration of the In Vitro diagnostic ELISA of autoimmune & infectious diseases
Human-diagnostic ELISAs
The ELISA tests listed below consist of 29 kits for In Vitro diagnosis of autoimmune diseases and 2 kits for the diagnosis of a Helicobacter pylori infection.
These ELISA kits are CE marked and come in the standard format of 96 analyses. They all use identical general reagents, namely sample buffer, wash buffer, substrate and stop solution. Hence, one and the same sample dilution may be used for multiple assays; e.g. for simultaneous determination of Cardiolipin IgG and IgM.
All solutions are ready to use (exception: 10-fold wash buffer concentrate) and color-coded. The conjugate color (red, yellow or green) indicates the Ig-class detected, IgG, IgA or IgM, respectively. The color intensity of the blue calibrators reflects their corresponding concentration. The negative control is green, the positive red.
The solid phase features break apart microwells for economic use of the kit. It is packed in a foil bag with zip closure, useful for partial consumption of the solid phase. The frame of the microwell plate displays the parameter name.
The sample is serum or plasma; 10 µL are required throughout. It is diluted 1:100 in the orange coloured sample buffer. 100 µL per microwell is the fixed volume of sample, conjugate, substrate and stop solution.
The tests share one common protocol: 3 x 30 minutes incubation at room temperature and without shaking. Wash steps: unvariably 4 x 350 µL per microwell.
The tests have been validated on the Dynex DS2 system and are accessible to other automated devices. The liquids come in polyethylene bottles with enough volume for automation. The detection system is HRP/TMB, read at 450nm.
The compact outer box is labelled on two adjacent sides for easy test kit identification in fridge stacks. Each product comes with printed Directions for Use (also downloadable), including a Summary Flow Chart on the back, and a kit lot-specific Certificate of Analysis. Most of the tests offer the option of qualitative or quantitative evaluation.
The excellent quality of these products is reflected by a very low number of complaints over the years. Since more than a decade, we regularly participate in several international ring trials (UK: Neqas, US: CAP, Germany: Instand and Referenzinstitut für Bioanalytik) with virtually no deviation of our results from the respective intended response.
The ELISA tests listed below consist of 29 kits for In Vitro diagnosis of autoimmune diseases and 2 kits for the diagnosis of a Helicobacter pylori infection.
These ELISA kits are CE marked and come in the standard format of 96 analyses. They all use identical general reagents, namely sample buffer, wash buffer, substrate and stop solution. Hence, one and the same sample dilution may be used for multiple assays; e.g. for simultaneous determination of Cardiolipin IgG and IgM.
All solutions are ready to use (exception: 10-fold wash buffer concentrate) and color-coded. The conjugate color (red, yellow or green) indicates the Ig-class detected, IgG, IgA or IgM, respectively. The color intensity of the blue calibrators reflects their corresponding concentration. The negative control is green, the positive red.
The solid phase features break apart microwells for economic use of the kit. It is packed in a foil bag with zip closure, useful for partial consumption of the solid phase. The frame of the microwell plate displays the parameter name.
The sample is serum or plasma; 10 µL are required throughout. It is diluted 1:100 in the orange coloured sample buffer. 100 µL per microwell is the fixed volume of sample, conjugate, substrate and stop solution.
The tests share one common protocol: 3 x 30 minutes incubation at room temperature and without shaking. Wash steps: unvariably 4 x 350 µL per microwell.
The tests have been validated on the Dynex DS2 system and are accessible to other automated devices. The liquids come in polyethylene bottles with enough volume for automation. The detection system is HRP/TMB, read at 450nm.
The compact outer box is labelled on two adjacent sides for easy test kit identification in fridge stacks. Each product comes with printed Directions for Use (also downloadable), including a Summary Flow Chart on the back, and a kit lot-specific Certificate of Analysis. Most of the tests offer the option of qualitative or quantitative evaluation.
The excellent quality of these products is reflected by a very low number of complaints over the years. Since more than a decade, we regularly participate in several international ring trials (UK: Neqas, US: CAP, Germany: Instand and Referenzinstitut für Bioanalytik) with virtually no deviation of our results from the respective intended response.
Our range of products
Product categories
- 03 Diagnostic Tests
- 03.02 Immunochemistry testing, immunology testing
- 03.02.01 Immuno assay testing
Immuno assay testing
- The TAPAS labelling Kit as alternative to the classical Biotin-Streptavidin system
- The Polystreptavidin R Surface Functionalization Kit
- BioTeZ offers a wide range of products and services in the field of Human Proteases Research, such as human MMPs, human ADAMTSor human HtrAs
- Autoimmune & Diagnostic ELISA (IVD)
- ELISA Development as a Service
- 03 Diagnostic Tests
- 03.02 Immunochemistry testing, immunology testing
- 03.02.02 Diagnostic rapid tests
Diagnostic rapid tests
- 03 Diagnostic Tests
- 03.05 Infectious immunology testing
- 03.05.01 Bacteriology testing
Bacteriology testing
- 03 Diagnostic Tests
- 03.07 Other diagnostics
Other diagnostics
- The TAPAS labelling Kit as alternative to the classical Biotin-Streptavidin system
- The Polystreptavidin R Surface Functionalization Kit
- BioTeZ offers a wide range of products and services in the field of Human Proteases Research, such as human MMPs, human ADAMTSor human HtrAs
- Autoimmune & Diagnostic ELISA (IVD)
- ELISA Development as a Service
Our products
Product category: Immuno assay testing, Diagnostic rapid tests, Bacteriology testing, Other diagnostics
The TAPAS labelling Kit as alternative to the classical Biotin-Streptavidin system
The TA-PAS System is a fast two component protein-ligand interaction system. One component is the small synthetic label called “TA-Label” (<1000 Da). The second component is the “PAS-Antibody” which binds the “TA-Label” with high specificity and efficiency.
The TA-PAS System supports a large number of applications including immobilization, enrichment or detection of antibodies, other proteins, primers and other nucleic acids. The TA-PAS System is a simple yet elegant system to link TA labelled molecules in assays by exploiting the high affinity to PAS capture antibody.
TA-PAS with double labelled molecules like primers, antibodies, Proteins
Immunoprecipitation: Immobilization of target molecules from cell culture or other biological samples
Target molecule enrichment
Application Example: Lateral Flow Test
Application of TA-PAS system in lateral flow in combination with a double labelling strategy
(antibody conjugated with Gold nanoparticles and TA-Label)
for immobilization at control line and streptavidin/biotin system for test line.
Advantages of the TA-PAS System compared with a clasical Biotin-Streptavidin System
No problems with double labelling strategies for lateral flow test
Application Example: Immobilisation of TA-labelled Molecules for Target molecule enrichment
The TA-PAS labelling kit allows antibodies, proteins, primer etc. to be covalently labelled with the TA-Label quickly and easily. The pre-activated TA-Label is designated for the coupling reaction with target molecules through the reaction with primary amino groups.
BioTeZ TA-PAS labelling kit includes the PAS-Antibody (capture antibody), all necessary reagents to label the target molecule with the TA-Labels and a clean-up column for purifying the coupling product (separation of unbound labels). Generally, the TA-Label is fully compatible with other labels and should work well.
For more details please read the product data sheet.
The TA-PAS System supports a large number of applications including immobilization, enrichment or detection of antibodies, other proteins, primers and other nucleic acids. The TA-PAS System is a simple yet elegant system to link TA labelled molecules in assays by exploiting the high affinity to PAS capture antibody.
- Fast binding universal detection system, e.g. lateral flow assay
- ELISA, it can be used as detection or coating component
- Immunohistochemistry (IHC)
- Western-, Southern- und Northern Blot
- Immunoaffinity chromatography
- Cell surface labelling etc.
TA-PAS with double labelled molecules like primers, antibodies, Proteins
Immunoprecipitation: Immobilization of target molecules from cell culture or other biological samples
Target molecule enrichment
Application Example: Lateral Flow Test
Application of TA-PAS system in lateral flow in combination with a double labelling strategy
(antibody conjugated with Gold nanoparticles and TA-Label)
for immobilization at control line and streptavidin/biotin system for test line.
Advantages of the TA-PAS System compared with a clasical Biotin-Streptavidin System
- The TA-PAS system allows a flexible, simple use in many applications
- Improvement of signal-noise ratio
- High affinity binding between TA-Label and PAS-Antibody (capture antibody)
- Fast Binding
- Low cross-reactivity
- Low non-specific binding
- TA-PAS interaction is artificial and not present in nature
- No problems with samples or proteins with biotin (e.g. Carboxylases, Histones)
- pH-stable
- Long shelf life time
- Reproducible results
No problems with double labelling strategies for lateral flow test
Application Example: Immobilisation of TA-labelled Molecules for Target molecule enrichment
The TA-PAS labelling kit allows antibodies, proteins, primer etc. to be covalently labelled with the TA-Label quickly and easily. The pre-activated TA-Label is designated for the coupling reaction with target molecules through the reaction with primary amino groups.
BioTeZ TA-PAS labelling kit includes the PAS-Antibody (capture antibody), all necessary reagents to label the target molecule with the TA-Labels and a clean-up column for purifying the coupling product (separation of unbound labels). Generally, the TA-Label is fully compatible with other labels and should work well.
For more details please read the product data sheet.
Product category: Immuno assay testing, Diagnostic rapid tests, Other diagnostics
The Polystreptavidin R Surface Functionalization Kit
The Polystreptavidin R Surface Functionalization Kit combines the excellent binding capacity with a high chemo and thermo stability.Additional pre-coating serves to improve the outcome and especially for very inert surfaces to ensure high quality coatings with Polystreptavidin R.
Polystreptavidin R provides product consistency and helps to reduce variability in diagnostic assays. Test it; make the comparison of BioTeZ Polystreptavidin R Coating Kit and surface activated beads or other surface activations. Typical applications where Polystreptavidin R is used: IVD assays, isolation and purification of biotinylated molecules, ligand coupling.
The BioTeZ Polystreptavidin R Coating Kit (Product Code: BTCK-MC) is useful for adsorptive coatings on microplates, beads, membranes etc. made of metal, nitrocellulose, ceramic, silicon, glass or plastics like Polystyrene, Polyethylene, Polypropylene, Polycarbonate, Poly(methyl methacrylate) PMMA or Polyamides with “Maximum Biotin Binding Capacity”.
Polystreptavidin R provides product consistency and helps to reduce variability in diagnostic assays. Test it; make the comparison of BioTeZ Polystreptavidin R Coating Kit and surface activated beads or other surface activations. Typical applications where Polystreptavidin R is used: IVD assays, isolation and purification of biotinylated molecules, ligand coupling.
The BioTeZ Polystreptavidin R Coating Kit (Product Code: BTCK-MC) is useful for adsorptive coatings on microplates, beads, membranes etc. made of metal, nitrocellulose, ceramic, silicon, glass or plastics like Polystyrene, Polyethylene, Polypropylene, Polycarbonate, Poly(methyl methacrylate) PMMA or Polyamides with “Maximum Biotin Binding Capacity”.
Product category: Immuno assay testing, Other diagnostics
BioTeZ offers a wide range of products and services in the field of Human Proteases Research, such as human MMPs, human ADAMTSor human HtrAs
BioTeZ offers a wide range of products and services in the field of Human Proteases Research, such as human MMPs (Matrix Metalloproteinases), human ADAMTS (A Disintegrin And Metalloprotease with Thrombospondin motifs) or human HtrAs (High temperature requirement factor A) to support drug development, especially therapeutic or neutralizing antibodies, antibody drug conjugates (ADC) or target screening and diagnostics, e.g. for preclinical phases, trials, pharmacokinetic (pk) and pharmacodynamic (pd) studies.
Proteases have relevance in the control of multiple biological processes in all living organisms. They regulate the activities of many proteins, modulate protein-protein interactions, create new bioactive molecules, contribute to the processing of cellular information and generate, transduce and amplify molecular signals. As a direct result of these multiple actions, proteases influence DNA replication and transcription, cell proliferation and differentiation, tissue morphogenesis and remodelling, heat shock and unfolded protein responses, angiogenesis, neurogenesis, ovulation, fertilization, wound repair, stem cell mobilization, haemostasis, blood coagulation, inflammation, immunity, autophagy, senescence, necrosis and apoptosis. Consistent with these essential roles of proteases in cell behaviour, survival and death of all organisms, alterations in proteolytic systems underlie multiple pathological conditions such as cancer, neurodegenerative disorders, inflammatory and cardiovascular diseases.
Our high quality proteins are produced in bacteria and insect cells (Baculovirus expression system) or are isolated from biological fluids.
Test kits, components and services for sensitive determination of protease concentration and protease activity fall within the scope of our Human Proteases Research. We combine the best elements of bead and ELISA technologies. This guarantees high sensitivity and specificity for many applications in various volumes and overcomes the problems caused by the interference of other proteins with the same molecular weight or the inhibition of specific protease detection due to the presence of an abundant amount of other proteins.
Proteases have relevance in the control of multiple biological processes in all living organisms. They regulate the activities of many proteins, modulate protein-protein interactions, create new bioactive molecules, contribute to the processing of cellular information and generate, transduce and amplify molecular signals. As a direct result of these multiple actions, proteases influence DNA replication and transcription, cell proliferation and differentiation, tissue morphogenesis and remodelling, heat shock and unfolded protein responses, angiogenesis, neurogenesis, ovulation, fertilization, wound repair, stem cell mobilization, haemostasis, blood coagulation, inflammation, immunity, autophagy, senescence, necrosis and apoptosis. Consistent with these essential roles of proteases in cell behaviour, survival and death of all organisms, alterations in proteolytic systems underlie multiple pathological conditions such as cancer, neurodegenerative disorders, inflammatory and cardiovascular diseases.
Our high quality proteins are produced in bacteria and insect cells (Baculovirus expression system) or are isolated from biological fluids.
Test kits, components and services for sensitive determination of protease concentration and protease activity fall within the scope of our Human Proteases Research. We combine the best elements of bead and ELISA technologies. This guarantees high sensitivity and specificity for many applications in various volumes and overcomes the problems caused by the interference of other proteins with the same molecular weight or the inhibition of specific protease detection due to the presence of an abundant amount of other proteins.
Product category: Immuno assay testing, Diagnostic rapid tests, Bacteriology testing, Other diagnostics
Autoimmune & Diagnostic ELISA (IVD)
The BioTeZ ELISA kits are CE marked and come in the standard format of 96 analyses. They all use identical general reagents, namely sample buffer, wash buffer, substrate and stop solution. Hence, one and the same sample dilution may be used for multiple assays; e.g. for simultaneous determination of Cardiolipin IgG and IgM. For more Information please contact us are drop by for a chat at the BioTeZ Stand.
Product category: Immuno assay testing, Diagnostic rapid tests, Bacteriology testing, Other diagnostics
ELISA Development as a Service
ELISA Development as s Service:
ELISA is an abbreviation for Enzyme-Linked Immuno Sorbent Assay. It is used to detect the enzyme-controlled reaction between antigen and antibody. This multi-purpose way of testing is, if used properly, a very sensitive verification procedure. Proteins, antibodies, biomarkers, viruses, hormones, toxins and pharmaceuticals can be analyzed and quantified this way.
For several years BioTeZ has distinguished know-how in the field of ELISA development and creates globally-recognized innovations repeatedly, for example the Therapeutic Drug Monitoring / Companion Diagnostic.
Make use of our knowledge in test development of Sandwich ELISAs, Indirect ELISAs or Competitive Immunoassays. In the latter case a labeled competitor antigen is used instead of a labeled antibody. The antigen is similar in structure to the analyte and also bonds to the antibody. This leads to competition between the analyte and the competitor for a binding space on the antibody.
We use different dyes and highly-active horseradish peroxidase for detection. We will be pleased to develop immunoassays for you, whether sandwich, indirect or competitive Assays.
At the premises of our sister company Steffens-Biotec, Ebringen (QMS: ISO 13485) we offer manufacturing in accordance with European IVD guidelines for CE labelled test kits and kit compounds.
ELISA is an abbreviation for Enzyme-Linked Immuno Sorbent Assay. It is used to detect the enzyme-controlled reaction between antigen and antibody. This multi-purpose way of testing is, if used properly, a very sensitive verification procedure. Proteins, antibodies, biomarkers, viruses, hormones, toxins and pharmaceuticals can be analyzed and quantified this way.
For several years BioTeZ has distinguished know-how in the field of ELISA development and creates globally-recognized innovations repeatedly, for example the Therapeutic Drug Monitoring / Companion Diagnostic.
Make use of our knowledge in test development of Sandwich ELISAs, Indirect ELISAs or Competitive Immunoassays. In the latter case a labeled competitor antigen is used instead of a labeled antibody. The antigen is similar in structure to the analyte and also bonds to the antibody. This leads to competition between the analyte and the competitor for a binding space on the antibody.
We use different dyes and highly-active horseradish peroxidase for detection. We will be pleased to develop immunoassays for you, whether sandwich, indirect or competitive Assays.
At the premises of our sister company Steffens-Biotec, Ebringen (QMS: ISO 13485) we offer manufacturing in accordance with European IVD guidelines for CE labelled test kits and kit compounds.
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Oct 30, 2019
Putting Ergot Alkaloids where they belong- in the history books
Putting Ergot Alkaloids where they belong- in the history books
The fungi Claviceps purpurea produces high concentrations of mycotoxins, called ergot alkaloids. Ergot alkaloids are complex molecule with a wide range of harmful biological activities. The new BioTeZ Ergot Alkaloids clean-up columns help to detect and quantify the mycotoxins in crops to prevent the toxification of human, livestock and domestic animals.
Ergot poisoning (ergotism) in humans and domestic animals cause hallucinations, the feeling of itchy, burning skin and can cause effects on neurotransmission and circulation. Ergotism is one of the oldest known human diseases caused by mycotoxins and references to grain diseases have been found in various books of the Bible (850-550 BC). In 1597 scientists at the University of Marburg observed that signs of ergotism often appeared after blighted rye grains were consumed and that ergotism was promoted by cold, damp growing seasons.
In 1630 it was observed that feeding of blighted grain to animals produced an illness similar to human ergotism and by the end of the 18th century poisoning was demonstrated in animals. Finally, in 1764, ergot was recognized as a fungus by Otto von Munchhausen (Schiff, 2006). Until the late 20th century, human and livestock exposure to ergot alkaloids was primarily through ingestion of “ergots,” which are spur-shaped or seed-like resting structures (sclerotia) of ergot fungi, the Claviceps species (Schardl, 2015).
In 1927 more than 11.000 cases of ergotism were reported from Sarapol (Russia). Another incident happened in 1951 when 230 villagers of the popular French tourist town of Pont Saint-Esprit were sickened after ingesting contaminated goods from a local baker. The victims became violently ill with symptoms of severe gastrointestinal upset, dramatic reduction in body temperature, hallucinations and euphoria. Note that ergotamine contains the same dopamine substructure which is also found in psychoactive drugs like lysergic one of the building blocks of the psychoactive drug lysergic acid diethylamide (LSD).
Although reports of ergotism in livestock vary from year-to-year according to rainfall and temperature, most cases occurs in the grain producing areas up today. An increase demand for biological produced products will cause a reduced use of fungicides which will lead to a higher need for ergot alkaloids monitoring in the next years. BioTeZ is already able to deliver a clean-up column of food and feed extracts for the reliable identification and accurate quantification of the predominant ergot alkaloid early in the food chain to avoid ergotism.
The extraction protocol of (Krska et al., 2008) is used in a modified version. Just briefly, a solid-phase extraction and clean-up method based on sodium-neutralized cation exchange to quantify 12 prioritised ergot alkaloids in rye flour and wheat germ oil by HPLC fluorescence analysis. The most abundant ergot alkaloids produced by fungus Claviceps purpurea comprise six pairs of epimeric ergot alkaloids (Köppen et al., 2013).
In summary ergot alkaloids still play a crucial role in the food and feed industries up to date. The reduction of fungicides in biological natural products will increase the need to monitor the ergot alkaloids in the futures food chain. BioTeZ offers an efficient Ergot Alkaloids clean-up column for the HPLC and mass spectrometry analysis, for any question and remarks, please contact us info@biotez.de directly.
References:
Köppen, R., Rasenko, T, Merkel, S., Mönch, B., and Koch, M. (2013). Novel solid-phase extraction for epimer-specific quantitation of ergot alkaloids in rye flour and wheat germ oil. J. Agric. Food Chem. 61, 10699–10707.
Krska, R., Stubbings, G., Macarthur, R., and Crews, C. (2008). Simultaneous determination of six major ergot alkaloids and their epimers in cereals and foodstuffs by LC-MS-MS. Anal Bioanal Chem 391, 563–576.
Schardl, C.L. (2015). Introduction to the Toxins Special Issue on Ergot Alkaloids. Toxins (Basel) 7, 4232–4237.
Schiff, P.L. (2006). Ergot and its alkaloids. Am J Pharm Educ 70, 98.
Oct 17, 2019
BioTeZ is starting to develop a new method for directly measuring the primary effect of drugs on their target molecule.
BioTeZ starts the project for the development of a companion diagnostic procedure. The aim is to determine the optimal drug dose for each patient to reduce side effects, to achieve the Therapeutic Window and to increase the effectiveness of the active ingredients by optimizing the dosage (Therapeutic Drug Monitoring).
The project is being funded on behalf of the State of Berlin through the Program for the Promotion of Research, Innovations and Technologies (Pro FIT) and partially funded by the European Regional Development Fund (ERDF). In the next two years, intensive work will be carried out to establish the Drug Primary Performance Monitoring Assay (DPPMA). Drug Primary Performance Monitoring Assay is characterized by the ability to simultaneously determine concentrations across binding curves of two molecules from different classes.
In addition, the application of various detection systems is tested. DPPMA is an excellent addition to BioTeZ’s therapeutic drug monitoring portfolio, with recoveryELISA and others. for Adalimumab and Evolocumab products include for the determination of protein-protein interactions.
“We are excited to begin this important project to improve therapies by optimizing the drug dose individually. With this project, we want to make it clear that in addition to the search for biomarkers, especially the identification of the appropriate active substance concentration in the patient, there is a great deal of catching up to do. Too often it happens that a patient does not reach a therapeutically meaningful concentration of active ingredient or that excessive levels of active ingredient are present. Without diagnostics, a treatment failure is otherwise quickly suspected. This problem of unintentional incorrect dosage can only be solved with intelligent diagnostics, “says Mr. Ahnert, Managing Director of BioTeZ Berlin-Buch GmbH.
The project is being funded on behalf of the State of Berlin through the Program for the Promotion of Research, Innovations and Technologies (Pro FIT) and partially funded by the European Regional Development Fund (ERDF). In the next two years, intensive work will be carried out to establish the Drug Primary Performance Monitoring Assay (DPPMA). Drug Primary Performance Monitoring Assay is characterized by the ability to simultaneously determine concentrations across binding curves of two molecules from different classes.
In addition, the application of various detection systems is tested. DPPMA is an excellent addition to BioTeZ’s therapeutic drug monitoring portfolio, with recoveryELISA and others. for Adalimumab and Evolocumab products include for the determination of protein-protein interactions.
“We are excited to begin this important project to improve therapies by optimizing the drug dose individually. With this project, we want to make it clear that in addition to the search for biomarkers, especially the identification of the appropriate active substance concentration in the patient, there is a great deal of catching up to do. Too often it happens that a patient does not reach a therapeutically meaningful concentration of active ingredient or that excessive levels of active ingredient are present. Without diagnostics, a treatment failure is otherwise quickly suspected. This problem of unintentional incorrect dosage can only be solved with intelligent diagnostics, “says Mr. Ahnert, Managing Director of BioTeZ Berlin-Buch GmbH.
Oct 17, 2019
BioTeZ established a novel therapeutic drug monitoring assay for Evolocumab and PCSK9
The first PCSK9 Inhibitors reach the market and BioTeZ just completed the development project for the therapeutic drug and antigen monitoring assay. The recoveryELISA® (RPE) allows the simultaneous quantification of the free PCSK9 and the free Evolocumab in one ELISA assay.
Proprotein convertase subtilisin/kexin type 9 (PCSK9) has medical importance since it directly interferes with the lipoprotein particles (LDL) homeostasis. PCSK9 binds to the lipoprotein particles receptor (LDLR) which transports fat molecules within the extracellular fluid. Agents which block PCSK9 can decrease the LDL particle concentrations in the blood and thereby prevent cardiovascular disease.
The first therapeutic monoclonal antibodies against PCSK9 Evolocumab and Alirocumab were approved by the U.S. Food and Drug Administration in 2015 for lowering LDL-particle concentrations when statins and other drugs were not sufficiently effective or poorly tolerated.
For Evolocumab two different dosing regimes are suggested 140 mg every two weeks or 420 mg once per month. By definition Therapeutic Drug Monitoring (TDM) is the clinical practice of measuring specific drugs to maintain a constant concentration in a patient's bloodstream, thereby optimizing individual dosage regimes (1).
“The recoveryELISA® RPE is the only method that simultaneously quantifies the free PCSK9, the PCSK9 neutralization rate and the free available therapeutic antibody Evolocumab in human serum samples during Evolocumab therapy. With our new recoveryELISA® RPE we support TDM studies and can contribute for a truly personalized dose finding strategy” explains Uwe Ahnert (CEO of BioTeZ).
A pilot study enrolled Evolocumab naïve patients suffering from residual cardiovascular disease, treated for the first time with evolocumab and followed for the next two injections. Serum samples were tested with the recoveryELISA© RPE to quantify free PCSK9 and free evolocumab simultaneously and compared these results with the lipid profile.
“We are glad to demonstrate the ability of the recoveryELISA© PCSK9 & Evolocumab for this important application in a clinical setting. Currently, we evaluate the data and we will start soon with writing up a publication on our findings” says Dr. Janko Brand (senior Scientist at BioTeZ).
Product specifications: The working range for PCSK9 is 0.04 - 0.35 µg/mL and Evolocumab 0.2- 24.0 µg/mL. Please use the serum samples at dilution of 1:10. Keep in mind that BioTeZ has also totalPCSK9 ELISA® (BTPCSK9-001) on stock for the quantification of PCSK9.
About BioTeZ: BioTeZ is a 1992 founded Biotech Company, developing and producing ELISA kits, protease activity kits, antibodies, diagnostics and oligonucleotides. BioTeZ has longstanding experience in the development of biochemical test procedures for research institutes, hospitals and laboratories worldwide. BioTeZ hold all relevant patents on the recoveryELISA© and the technology was previously established for Omalizumab/IgE (2), Infliximab/TNFa (3), Adalimumab/TNFa (4) and customized for clients involved in early biological development projects from the pharmaceutical industry.
References:
1. Kang J-S, Lee M-H. Overview of Therapeutic Drug Monitoring. Korean J Intern Med [Internet] 2009 [cited 2018 Sep 21];24:1-10. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2687654/
2. Gericke J, Metz M, Ohanyan T, Weller K, Altrichter S, Skov PS, et al. Serum autoreactivity predicts time to response to omalizumab therapy in chronic spontaneous urticaria. J Allergy Clin Immunol 2017;139:1059-1061.e1.
3. Strohner P, Staatz A, Sarrach D, Steiss J-O, Becher G. The recoveryELISA--a newly developed immunoassay for measurement of therapeutic antibodies and the target antigen during antibody therapy. Clin Chem Lab Med 2012;50:1263-9.
4. Zänker M, Becher G, Arbach O, Maurer M, Stuhlmüller B, Schäfer A, et al. Improved adalimumab dose decision with comprehensive diagnostics data. Clin Exp Rheumatol 2018;36:136-9.
Proprotein convertase subtilisin/kexin type 9 (PCSK9) has medical importance since it directly interferes with the lipoprotein particles (LDL) homeostasis. PCSK9 binds to the lipoprotein particles receptor (LDLR) which transports fat molecules within the extracellular fluid. Agents which block PCSK9 can decrease the LDL particle concentrations in the blood and thereby prevent cardiovascular disease.
The first therapeutic monoclonal antibodies against PCSK9 Evolocumab and Alirocumab were approved by the U.S. Food and Drug Administration in 2015 for lowering LDL-particle concentrations when statins and other drugs were not sufficiently effective or poorly tolerated.
For Evolocumab two different dosing regimes are suggested 140 mg every two weeks or 420 mg once per month. By definition Therapeutic Drug Monitoring (TDM) is the clinical practice of measuring specific drugs to maintain a constant concentration in a patient's bloodstream, thereby optimizing individual dosage regimes (1).
“The recoveryELISA® RPE is the only method that simultaneously quantifies the free PCSK9, the PCSK9 neutralization rate and the free available therapeutic antibody Evolocumab in human serum samples during Evolocumab therapy. With our new recoveryELISA® RPE we support TDM studies and can contribute for a truly personalized dose finding strategy” explains Uwe Ahnert (CEO of BioTeZ).
A pilot study enrolled Evolocumab naïve patients suffering from residual cardiovascular disease, treated for the first time with evolocumab and followed for the next two injections. Serum samples were tested with the recoveryELISA© RPE to quantify free PCSK9 and free evolocumab simultaneously and compared these results with the lipid profile.
“We are glad to demonstrate the ability of the recoveryELISA© PCSK9 & Evolocumab for this important application in a clinical setting. Currently, we evaluate the data and we will start soon with writing up a publication on our findings” says Dr. Janko Brand (senior Scientist at BioTeZ).
Product specifications: The working range for PCSK9 is 0.04 - 0.35 µg/mL and Evolocumab 0.2- 24.0 µg/mL. Please use the serum samples at dilution of 1:10. Keep in mind that BioTeZ has also totalPCSK9 ELISA® (BTPCSK9-001) on stock for the quantification of PCSK9.
About BioTeZ: BioTeZ is a 1992 founded Biotech Company, developing and producing ELISA kits, protease activity kits, antibodies, diagnostics and oligonucleotides. BioTeZ has longstanding experience in the development of biochemical test procedures for research institutes, hospitals and laboratories worldwide. BioTeZ hold all relevant patents on the recoveryELISA© and the technology was previously established for Omalizumab/IgE (2), Infliximab/TNFa (3), Adalimumab/TNFa (4) and customized for clients involved in early biological development projects from the pharmaceutical industry.
References:
1. Kang J-S, Lee M-H. Overview of Therapeutic Drug Monitoring. Korean J Intern Med [Internet] 2009 [cited 2018 Sep 21];24:1-10. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2687654/
2. Gericke J, Metz M, Ohanyan T, Weller K, Altrichter S, Skov PS, et al. Serum autoreactivity predicts time to response to omalizumab therapy in chronic spontaneous urticaria. J Allergy Clin Immunol 2017;139:1059-1061.e1.
3. Strohner P, Staatz A, Sarrach D, Steiss J-O, Becher G. The recoveryELISA--a newly developed immunoassay for measurement of therapeutic antibodies and the target antigen during antibody therapy. Clin Chem Lab Med 2012;50:1263-9.
4. Zänker M, Becher G, Arbach O, Maurer M, Stuhlmüller B, Schäfer A, et al. Improved adalimumab dose decision with comprehensive diagnostics data. Clin Exp Rheumatol 2018;36:136-9.
Oct 17, 2019
BioTeZ established a novel surface functionalization kit -the TAPAS system.
The strong interaction between streptavidin and biotin is widely utilized in biotechnological applications (1). The new TAPAS label was developed to extend the portfolio for assay manufactured and will be valuable for its applications across a wide range of biotechnological areas.
To meet the big demand BioTeZ introduced in 2008 the poly-streptavidin a crosslinked streptavidin polymer with increased biotin binding capacity widely used for lateral flow test applications (2). Now is 2018 BioTeZ released a novel surface functionalization platform the TAPASTM Labeling Kit. This kit or labeling service allows an effective labeling a target molecules and detection with specific antibody.
The BioTeZ TAPASTM Labeling Kit was developed to extend the portfolio for assay manufactured and will be valuable for its applications across a wide range of areas.The BioTeZ TAPASTM Labeling Kit is a two component protein-ligand interaction system. One component is the small 1000 Dalton synthetic label called “TA-label”.
The second component is the “PAS-Antibody” which binds the “TA-label” with high specificity and efficiency (Figure 1). The BioTeZ TAPASTM Labeling Kit or labelling service allows antibodies, proteins, primer etc. to be covalently labelled with the TA-label. The pre-activated TA-label is designated for the coupling with the target molecules through the reaction with primary amino groups which allows also double labelling strategies ideal for lateral flow test development projects.
“With the BioTeZ TAPASTM Labeling Kit we react on the high demand on surface functionalization technologies for the diagnostic field. With our new TAPASTM Labeling Kit we support lateral flow test manufacturing and contribute to a more convince to develop a new point of care technology” explains Dr. Janko Brand (Senior Scientist at BioTeZ).
The BioTeZ TAPASTM Labeling Kit supports a large number of applications including immobilization, enrichment or detection of antibodies, other proteins and nucleic acids. It can be flexibly combined with various target molecules and detection systems such as fluorescent, luminescent, gold or carbon black (3).
Many commercial immunoassays employ the high affinity interaction between biotin and streptavidin as part of the analyte capture mechanism. As such, these immunoassays are subject to an interference if biotin is part of the diet (4). The new TAPAS Label is biotin free and will reduce the background and thereby increase the sensitivity for many applications.
“We are glad to demonstrate the ability of the TAPASTM Labeling Kit for this important application in the coating and surface functionalization. Currently, we are in dialog with all major distributors and prepare a worldwide roll out of the BioTeZ TAPASTM Labeling Kit” says Uwe Ahnert (CEO BioTeZ).
The advantages of the BioTeZ TAPASTM Labeling Kit compared to conventional functionalization reagents are the improved signal noise ratio compared to streptavidin and poly-streptavidin. The high affinity binding between TA-Tag and PAS-Capture antibody leads to rapid binding with low cross-reactivity. Since the TA-PAS label is artificial and not present in nature non-specific binding were detected.
Product specifications: The BioTeZ TAPASTM Labeling Kit includes the PAS capture antibody, all necessary reagents to label the target molecule with the TA-labels and clean-up columns for purifying the coupling product. For double labelling TA-label and gold nanoparticles it is recommended to do TA-labelling procedure first.
About BioTeZ: BioTeZ is a 1992 founded Biotech Company, developing and producing ELISA kits, protease activity kits, antibodies, diagnostics and oligonucleotides. BioTeZ has longstanding experience in the development of biochemical test procedures for research institutes, hospitals and laboratories worldwide. BioTeZ holds all relevant patents on the poly-streptavidin and TAPAS label production and distribution.
References
1. Zhang M, Biswas S, Deng W, Yu H. The Crystal Structure of Monovalent Streptavidin. Sci Rep 2016;6:35915.
2. Wang X, Du M, Mao G, Zheng J, Chen J, Ji X, et al. The synthesis of a smart streptavidin-functionalized poly(N-isopropylacrylamide) composite and its application in the separation and detection of virus nucleic acid. Talanta 2018;181:73-9.
3. Bové H, Steuwe C, Fron E, Slenders E, D’Haen J, Fujita Y, et al. Biocompatible Label-Free Detection of Carbon Black Particles by Femtosecond Pulsed Laser Microscopy. Nano Lett 2016;16:3173-8.
4. Gifford JL, de Koning L, Sadrzadeh SMH. Strategies for mitigating risk posed by biotin interference on clinical immunoassays. Clin Biochem 2018;
To meet the big demand BioTeZ introduced in 2008 the poly-streptavidin a crosslinked streptavidin polymer with increased biotin binding capacity widely used for lateral flow test applications (2). Now is 2018 BioTeZ released a novel surface functionalization platform the TAPASTM Labeling Kit. This kit or labeling service allows an effective labeling a target molecules and detection with specific antibody.
The BioTeZ TAPASTM Labeling Kit was developed to extend the portfolio for assay manufactured and will be valuable for its applications across a wide range of areas.The BioTeZ TAPASTM Labeling Kit is a two component protein-ligand interaction system. One component is the small 1000 Dalton synthetic label called “TA-label”.
The second component is the “PAS-Antibody” which binds the “TA-label” with high specificity and efficiency (Figure 1). The BioTeZ TAPASTM Labeling Kit or labelling service allows antibodies, proteins, primer etc. to be covalently labelled with the TA-label. The pre-activated TA-label is designated for the coupling with the target molecules through the reaction with primary amino groups which allows also double labelling strategies ideal for lateral flow test development projects.
“With the BioTeZ TAPASTM Labeling Kit we react on the high demand on surface functionalization technologies for the diagnostic field. With our new TAPASTM Labeling Kit we support lateral flow test manufacturing and contribute to a more convince to develop a new point of care technology” explains Dr. Janko Brand (Senior Scientist at BioTeZ).
The BioTeZ TAPASTM Labeling Kit supports a large number of applications including immobilization, enrichment or detection of antibodies, other proteins and nucleic acids. It can be flexibly combined with various target molecules and detection systems such as fluorescent, luminescent, gold or carbon black (3).
Many commercial immunoassays employ the high affinity interaction between biotin and streptavidin as part of the analyte capture mechanism. As such, these immunoassays are subject to an interference if biotin is part of the diet (4). The new TAPAS Label is biotin free and will reduce the background and thereby increase the sensitivity for many applications.
“We are glad to demonstrate the ability of the TAPASTM Labeling Kit for this important application in the coating and surface functionalization. Currently, we are in dialog with all major distributors and prepare a worldwide roll out of the BioTeZ TAPASTM Labeling Kit” says Uwe Ahnert (CEO BioTeZ).
The advantages of the BioTeZ TAPASTM Labeling Kit compared to conventional functionalization reagents are the improved signal noise ratio compared to streptavidin and poly-streptavidin. The high affinity binding between TA-Tag and PAS-Capture antibody leads to rapid binding with low cross-reactivity. Since the TA-PAS label is artificial and not present in nature non-specific binding were detected.
Product specifications: The BioTeZ TAPASTM Labeling Kit includes the PAS capture antibody, all necessary reagents to label the target molecule with the TA-labels and clean-up columns for purifying the coupling product. For double labelling TA-label and gold nanoparticles it is recommended to do TA-labelling procedure first.
About BioTeZ: BioTeZ is a 1992 founded Biotech Company, developing and producing ELISA kits, protease activity kits, antibodies, diagnostics and oligonucleotides. BioTeZ has longstanding experience in the development of biochemical test procedures for research institutes, hospitals and laboratories worldwide. BioTeZ holds all relevant patents on the poly-streptavidin and TAPAS label production and distribution.
References
1. Zhang M, Biswas S, Deng W, Yu H. The Crystal Structure of Monovalent Streptavidin. Sci Rep 2016;6:35915.
2. Wang X, Du M, Mao G, Zheng J, Chen J, Ji X, et al. The synthesis of a smart streptavidin-functionalized poly(N-isopropylacrylamide) composite and its application in the separation and detection of virus nucleic acid. Talanta 2018;181:73-9.
3. Bové H, Steuwe C, Fron E, Slenders E, D’Haen J, Fujita Y, et al. Biocompatible Label-Free Detection of Carbon Black Particles by Femtosecond Pulsed Laser Microscopy. Nano Lett 2016;16:3173-8.
4. Gifford JL, de Koning L, Sadrzadeh SMH. Strategies for mitigating risk posed by biotin interference on clinical immunoassays. Clin Biochem 2018;
About us
Company details
The BioTeZ Berlin-Buch GmbH is a Biotechnology company founded in 1992. BioTeZ is partner of research institutions, the pharmaceutical industry and laboratory diagnostics.
Our sister company Steffens-Biotec, Ebringen (ISO 13485) is specialised in manufacturing CE in-vitro diagnostics.
We are happy to talk about your ideas and needs in the fields of:
Our sister company Steffens-Biotec, Ebringen (ISO 13485) is specialised in manufacturing CE in-vitro diagnostics.
We are happy to talk about your ideas and needs in the fields of:
- Target enrichment with Immunoaffinity chromatography
- Monitoring Drug Efficiency with Antibodies as Drugs (TDM)
- Autoimmune & Diagnostic ELISA (CE/ IVD)
- ELISA & LFA Development
- Surfaces functionalization with Poly-Streptavidin and TAPAS Systems (Biotin free)
- Measurement of Protease Activity
- Oligonucleotides and Aptamers
Company data
Sales volume1-9 Mio US $
Export content> 75%
Number of employees20-49
Foundation1992
Area of businessDiagnostics