EXPEDEON, LTD of Cambridgeshire at MEDICA 2019 in Düsseldorf -- MEDICA - World Forum for Medicine

EXPEDEON, LTD

25 Norman Way, Over, CB24 5QE Cambridgeshire
United Kingdom of Great Britain and Northern Ireland

This company is co-exhibitor of
Gambica Association Limited

Hall map

MEDICA 2019 hall map (Hall 1): stand H09-1

Fairground map

MEDICA 2019 fairground map: Hall 1

Our range of products

Product categories

  • 03  Diagnostic Tests
  • 03.02  Immunochemistry testing, immunology testing
  • 03.02.01  Immuno assay testing

Our products

Product category: Immuno assay testing

CaptSure DIY ELISA

Powered by Lightning-Link®
 
Immunoassays are the gold-standard method for the detection of proteins and biomarkers in discovery processes. However, ELISA development usually involves costly plate coating, antibody pair screening, long development and optimization times, and variable success rates. These limitations are now solved by CaptSure DIY ELISA.

CaptSure DIY ELISA is the latest cutting-edge ELISA kit with a built-in system for antibody labeling, enabling the rapid and easy development of in-house ELISAs using any antibody pair while eliminating the need to perform plate coating procedures.

Furthermore, the CaptSure DIY ELISA enables you to reduce the amount of capture antibody required, the ability to measure different targets on the same plate with different assay antibody pairs, and streamline your ELISA to a single-wash format if desired.

The CaptSure DIY ELISA optimized protocol results in faster ELISA development, reduced overall costs, and a greater assay flexibility to increase the research output with a reduced chance of error.

How does CaptSure DIY ELISA work?

CaptSure DIY ELISA is powered by the combination of Expedeon’s proprietary CaptSure and Lightning-Link technologies.

Unlike standard ELISA systems, the CaptSure DIY ELISA kit has a ready-to-go CaptSure assay plate, pre-coated with our CaptSure antibody, which is specific for the CaptSure peptide. The capture antibody is quickly and easily conjugated with the Lightning-Link CaptSure peptide supplied within the kit.  Once added to the plate, the interaction between the CaptSure antibody and the CaptSure peptide immobilizes the immuno-complex to the plate.

The detection antibody is similarly conjugated with HRP using the Lightning-Link HRP conjugation reagent, also supplied in the kit. Thus, with minimal hands-on time, you can set up both of your antibodies to ensure the best performance of your ELISA.

Lightning-Link is the world’s fastest conjugation technology to label antibodies and proteins by targeting free amine groups. As amine groups are ubiquitous and numerous in antibodies, any antibody can be labeled with the Lightning-Link CaptSure peptide and Lightning-Link HRP kits.

This labeling protocol is comprised of just a few simple steps:

Resuspend the lyophilized Lightning-Link CaptSure peptide and Lightning-Link HRP with the solution of capture and detection antibodies, respectively;
Incubate each at room temperature for the specified times;
Quench the reactions, and your labeled capture and detection antibodies are ready to use for the ELISA.

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Product category: Immuno assay testing

ELISA-ONE™

ELISA-ONE are next generation ready to use ELISA kits for the fast detection of key intracellular proteins that undergo phosphorylation in major signaling pathways.

Protein phosphorylation plays a crucial role during intracellular signaling, but the availability of optimized and validated antibody pairs often presents a critical bottleneck for cell signaling research. ELISA-ONE kits are powered by our CaptSure technology and are comprised of precoated plates and an optimized pair of capture and detection antibodies that recognize either specific phosphoepitopes or invariant epitopes on each target.

What’s Different About ELISA-ONE?
Unlike most other ELISA protocols that require long incubation times and multiple wash steps, ELISA-ONE can be performed in no more than 90 minutes, with a single incubation and washing steps, whilst maintaining the same performance, sensitivity, and dynamic range of conventional protocols. Furthermore, the technology behind ELISA-ONE is particularly effective at eliminating the issues related to biotin interference that generally occurs in complex cell lysates.

The simplicity and efficiency of ELISA-ONE ultimately translates into faster assay setup and analysis, improved performance in complex samples and reduced cost.

ELISA-ONE Workflow

The sample, the capture antibody tagged with CaptSure peptide, and the HRP-labeled detection antibody are added to the CaptSure antibody coated plate in one single step and incubated for 60 minutes. The immunocomplex formed by the analyte within the sample and the antibody pair is anchored to the plate via the interaction between the CaptSure antibody immobilized onto the plate and the CaptSure peptide conjugated to the capture antibody.

Following a washing step, unbound material is washed away and an HRP substrate (TMB) is added to the plate for colorimetric detection. The color development, due to the oxidation of TMB, is measured at 450nm and directly correlates with the amount of analyte in the sample.

The whole process takes no more than 90 minutes, cutting hands-on time in half and reducing incubation times by three times compared with conventional ELISA protocols.
 

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Product category: Immuno assay testing

ATPase & GTPase Assay Kits

Methods for measuring inorganic phosphate (Pi) using dyes such as Malachite Green are well known, however the dye–Pi complexes are prone to precipitation and the reagents are very acidic, which can lead to nonenzymatic hydrolysis of many phosphorylated substrates.

At the heart of Expedeon’s range of phosphate detection products is PiColorLockTM a unique phosphate detection reagent that overcomes these common issues. The formulation of PiColorLockTM affords enhanced assay linearity, dynamic range and color stability, and it is also possible to work with unstable substrates that give high nonenzymatic background signals with other acidic dye based detection reagents.

PiColorLockTM can be purchased singularly or as part of our ATPase and GTPase assay kits.

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About us

Company details

What we do
Expedeon is a UK-based company specializing in development of next-generation tools for biological research, diagnostics, and drug discovery.

Our hallmark is to provide technologies that speed up our clients’ research. Expedeon has a breadth of products, including phosphoprotein ELISAs, rapid antibody labeling kits for HRP, biotin and other detection molecules, as well as precast electrophoresis gels and associated equipment.

With offices, laboratories and manufacturing facilities in the USA, UK, Australia, Spain and Singapore, we are always within reach for your scientific needs.

Why we stand out
Our stringent QC processes promise high-quality products with excellent batch-to-batch reproducibility
Our products are rated 4.9/5 by our customers
Our technical support is rated over 9/10 by customers
We also offer tailored products and services to meet your requirements
We are ISO accredited to ensure high quality products and support
We dispatch 90% of orders placed within 48 hours

Supporting our customers

Fast global shipping
Delivery to over 200 countries
Expert technical support – response within 24 hours
In-house expertise supporting you through your application
Tailored custom services
Easy ordering online or via phone, email or fax
Online technical documentation and protocols
Online educational resources

Find us
Expedeon provides product, services and technical support to researchers and manufacturers globally. Our internal manufacturing and logistics facilities in the UK and US and our vast distribution network enable us to deliver fast and efficient delivery to customers worldwide.

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