engine GmbH of Hennigsdorf at MEDICA 2018 in Düsseldorf -- MEDICA - World Forum for Medicine

engine GmbH

Neuendorfstr. 17, 16761 Hennigsdorf
Germany
Telephone +49 3302 5519983
Fax +49 3302 5519910
info@engine-gmbh.de

This company is co-exhibitor of
DiagnostikNet-BB Netzwerk Diagnostik Berlin-Brandenburg e.V.

Hall map

MEDICA 2018 hall map (Hall 3): stand G52

Fairground map

MEDICA 2018 fairground map: Hall 3

Our range of products

Product categories

  • 03  Diagnostics
  • 03.02  Immunochemistry testing, immunology testing
  • 03.02.01  Immuno assay testing

Immuno assay testing

Our products

Product category: Immuno assay testing

engine Protein Arrays

engine offers different types of Protein Macroarrays. The corresponding cDNA expression libraries were validated and advanced based on more than fifteen years of experience in the field.

hEX1 engine Protein Macroarray
The hEX1 protein expression library was constructed by preparing cDNA from human fetal brain poly(A)+ RNA, using oligo (dT)-priming. The resulting fragments (larger than 500 bp) were cloned into a modified expression vector (pQE30NST) allowing protein expression to be induced by addition of IPTG to the growth medium. The resulting expressed proteins include an N-terminal RGS-HIS-Tag, which can be used for detection and purification purposes. 193,536 clones were picked, spotted and induced on PVDF-membranes and thereafter assayed for protein expression using an anti-HIS-antibody. Based on this screen, 37,830 putative expression clones were selected and re-arrayed into 384 well microtiter plates which constitute the hEX1-library. Spotted on high-density protein filters on two PVDF-membranes, the library is available as hEX1 part 1 and part 2 (Büssow et al., NAR1998). Most clones of this library have been 5’tag sequenced and annotated. The clone collection contains full-length as well as shorter cDNA clones representing full-length along with partial proteins. Some clones express artificial sequences derived from cDNA-construct as well as 5’ and 3’ UTRs.

hEXselect engine Protein Macroarray
The hEXselect protein expression library was derived from the hEX1 library by in silico analysis and re-arraying. Genes which were available in a high clone number were reduced to decrease redundancy. Expressed proteins, which were available only as a single copy were doubled for successive production of high density protein macroarrays. The hEXselect protein expression library contains 23,806 clones. Like the hEX1-library, it comprises full-length as well as shorter cDNA clones. All clones have been 5’ tag sequenced and are fully annotated. The size of the low redundancy collection allows a complete spotting in duplicates onto a single Protein Array, which saves processing time and hybridisation probe volume. The majority of the clones represent partial proteins, which may include artificial protein sequences corresponding to the 5’UTR sequences, with one third matching to the human proteome. Protein Arrays are delivered with an accompanying annotation table and respective spotting positions.

UniPEx engine Protein Macroarray
The UniPEx protein expression library consists of 2 Arrays representing clones in 2 different modified vectors. In total, more than 100,000 sequenced clones from different protein expression libraries (human fetal brain, T-cells, lung) were analysed in depth for their coding potential. After in-frame analysis only clones with a confirmed in-frame ORF were selected and redundancy with respect to clones per gene was minimized to less than 3 fold. In total, 15,456 UniPEx clones represent 7,390 distinct human proteins.

Custom-made engine Protein Arrays
can be generated from subsets of these libraries in the range of 500 to 13,000 clones spotted in duplicates. The customer provides us with a list of desired clones/genes/fragments or even his own clones. Clones of the customer may have to undergo sub cloning, in order to be able to express protein on the array. We will then analyse the set and, if wanted, may offer some more/different clones to be placed on the array. We also can provide suitable controls (e.g. house keeping genes) to be included on the array. In agreement with the customer, we can then set up the custom-made Protein Array of the desired size.

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About us

Company details

We are a biotechnology company, located with headquarters in Hennigsdorf just outside Berlin and a production site in Berlin-Buch. Directions can be found here.

We provide our customers with a range of products in the field of protein array technology, enabling them to perform high throughput and multiplexed assays, by addressing up to 2 × 27,648 in situ expressed proteins in a single experiment.

We offer numerous engine Protein Macroarrays presenting human protein collections derived from different tissues. In addition, we offer customized protein arrays according to the individual needs of our customers. Sizes of the customized arrays range from ∼500 up to 13,000 individual clones, printed in duplicates.

Using our engine Protein Macroarrays, protein interactions with many types of molecules can be investigated. Our arrays can be applied to determine antibody profiles of complex sera, cross-reactivity of monoclonal antibodies, protein-protein interaction pairs, DNA/RNA binding proteins, etc.

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