This kit is for classification of hematocytes in blood and marrow.
Wright-Giemsa Stain, as modified from Romanowsky Stain technique, is intended for staining smears of blood and marrow.
Staining of cells involves physical adsorption and chemical affinity which allows stain to penetrate and remain wiithin cells. Becasuse each cell and its components are different in chemical composition, their affinity to acid stain (Eosin) and alkaline stain (Methylene blue) of this kit varies significantly. After staining the smear with Wright's Stain, different types of cells will appear in different colors. Consequently, one can identify cells based on their stain color, shape and other physical characteristics.
1. Add Wright-Giemsa Solution A (about 0.5~0.8ml) to the smear and allow to cover the whole specimen. Stain for 1 min.
2. Add Wright-Giemsa Solution B (2~3 times of Solution A) onto Solution A and mix thoroughtly with a rubber pipette bulb. Allow to stain for 5~10mins.
3. Rinse with water gently, dry and examine the finished slide under a microscope.