PCR Biosystems Ltd. of London at MEDICA 2018 in Düsseldorf -- MEDICA - World Forum for Medicine

PCR Biosystems Ltd.

Aztec House, 397-405 Archway Road, N6 4ER London
United Kingdom of Great Britain and Northern Ireland
Telephone +44 203930 8101
Fax +44 207681 2186
info@pcrbio.com

This company is co-exhibitor of
Gambica Association Limited

Hall map

MEDICA 2018 hall map (Hall 3A): stand 3AD02-4

Fairground map

MEDICA 2018 fairground map: Hall 3A

Contact

Alex Wilson

Co-Founder and Business Developent Manager

Phone
+44 (0) 203 930 8101
+44 (0) 7717 874 735

Email
alex@pcrbio.com

Mary Caudron

Marketing and Communications Manager

Phone
+44 (0) 203 930 8010
+44 (0) 7506 862158

Email
mary@pcrbio.com

Our products

Product category: Polymerase chain reaction (PCR)

qPCRBIO SyGreen Blue Mix

qPCRBIO SyGreen Blue Mix contains a non-reactive blue dye for easy sample visualisation and greater precision for your qPCR experiments. Our blue mix is particularly useful when loading small volumes, using white plates or when foolproof qPCR setup is required. Together with advanced enzyme, hot start and reaction buffer technology, we offer market-leading sensitivity and reproducibility with enhanced pipetting accuracy.

More Less

Product category: Polymerase chain reaction (PCR)

UltraScript 2.0 Reverse Transcriptase

UltraScript 2.0 Reverse Transcriptase is a robust and highly thermostable modified MMLV reverse transcriptase engineered for superior cDNA synthesis speed, yield and representation from a wide range of RNA sample types, including GC-rich and low abundance templates.

The enhanced thermostability of UltraScript 2.0 Reverse Transcriptase (RTase) allows reaction temperatures of over 55°C to be used, giving improved specificity, higher cDNA yields, and more full length cDNA product. UltraScript 2.0 RTase is capable of efficient reverse transcription of the most challenging templates, including GC- rich and highly structured transcripts. UltraScript 2.0 kits can be used with 20pg to 3.5μg total RNA or oligo(dT) purified mRNA. The RTase is pre-blended with an advanced RNase inhibitor to prevent degradation of RNA by contaminating RNase.

More Less

Product category: Polymerase chain reaction (PCR)

PCRBIO HS Taq DNA Polymerase and Mixes

PCRBIO HS Taq DNA Polymerase is an advanced antibody-mediated hot start DNA polymerase designed for fast, highly specific PCR. Proprietary antibodies inhibit polymerase activity until the initial activation step at 95°C, preventing the formation of primer dimers and non-specific products, giving improved specificity and sensitivity compared to other methods. Whether you need a hot start assay for high-throughput, automated reaction setup or the detection of a low copy number template, PCR Biosystems offers you a robust industry-leading enzyme to meet your needs.

PCRBIO HS Taq DNA Polymerase is also available as a convenient, ready-to-use 2x mix containing all reaction components except primers and template. PCRBIO HS Taq Mix Red contains a preloaded red dye for direct gel loading.

More Less

Product category: Polymerase chain reaction (PCR)

qPCRBIO cDNA Synthesis Kit

High quality cDNA synthesis is essential for downstream real-time PCR analysis and successful expression studies. qPCRBIO cDNA Synthesis Kit is an easy-to-use 2 tube system specifically developed to generate cDNA for use in real-time PCR. The thermostable reverse transcriptase, buffer system and optimised blend of random hexamers with anchored oligo(dT) primers provide unbiased, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations.

More Less

Product category: Polymerase chain reaction (PCR)

PCRBIO Rapid Extract PCR Kit

PCRBIO Rapid Extract PCR Kit combines rapid DNA extraction with fast, highly specific DNA amplification in a convenient, easy-to-use format. Eliminate the need for laborious and time-consuming DNA extraction methods with this simple, integrated extraction and amplification PCR kit powered by the latest advances in hot start polymerase technology.

PCRBIO Rapid Extract PCR Kit is suited to solid tissue (such as mouse tail or mouse ear) hair follicle, buccal swab, mammalian blood and FFPE tissue. Sample processing is simplified and contamination risks minimised as DNA extraction is performed in a single tube, removing the need for multiple washing steps.

More Less

Product category: Polymerase chain reaction (PCR)

qPCRBIO SyGreen Mix

qPCRBIO SyGreen Mix combines a proprietary non-inhibiting intercalating dye with the latest advances in polymerase technology and buffer chemistry to give you fast, highly sensitive and reproducible real-time PCR. Antibody-mediated hot start technology prevents the formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity with minimal or no optimisation required.

qPCRBIO SyGreen Mix can be used to reliably quantify any DNA template including genomic, cDNA and viral sequences, and is able to detect extremely low copy number targets with the highest efficiency.

More Less

Product category: Polymerase chain reaction (PCR)

qPCRBIO Probe Mix

qPCRBIO Probe Mix is a universal probe kit designed to give superior sensitivity and specificity in all probe-based real-time PCR assays including TaqMan®, Scorpions® and molecular beacon probes. By combining antibody-mediated hot start technology with the latest advances in buffer chemistry we offer market-leading performance with minimal or no optimisation required. qPCRBIO Probe Mix can be used to reliably detect extremely low copy number targets and quantify any DNA template including genomic, cDNA and viral sequences. The enhanced sensitivity of qPCRBIO Probe Mix makes it the perfect choice for multiplexing.

More Less

Product category: Polymerase chain reaction (PCR)

qPCRBIO Probe Blue Mix

qPCRBIO Probe Blue Mix is a universal probe kit that includes a non-reactive blue dye for easy sample visualisation and enhanced pipetting accuracy. Whether your application is for a singleplex or multiplex expression study or a diagnostic assay, qPCRBIO Probe Blue Mix is the robust choice for all your probe-based real-time PCR needs.

More Less

Product category: Polymerase chain reaction (PCR)

qPCRBIO Genotyping Mix

qPCRBIO Genotyping Mix is designed for fast, accurate and reproducible allelic discrimination for use in TaqMan® and other dual-labelled probe-based genotyping assays. Antibody-mediated hot start technology combined with advanced buffer chemistry gives highly sensitive and specific real-time PCR resulting in superior allele clustering. qPCRBIO Genotyping Mix is able to accurately call class I to class IV mutations and is compatible with LNA and PNA probes for more stringent allele calling.

More Less

Product category: Polymerase chain reaction (PCR)

qPCRBIO HRM Mix

High Resolution Melt is a powerful post-PCR technique for the analysis of mutations, polymorphisms and epigenetic differences in double stranded DNA samples. Samples are characterized based on DNA strand dissociation behavior as temperature is increased in the presence of a fluorescent dye. qPCRBIO HRM Mix uses SyGreen 2, a 3rd generation non-PCR inhibiting saturating dye which produces ultra-sensitive melt profiles capable of discriminating class I to IV mutations as well as CpG methylation differences.

More Less

Product category: Polymerase chain reaction (PCR)

qPCRBIO SyGreen 1-Step Detect | 1-Step Go

qPCRBIO SyGreen 1-Step Kits have been designed for fast, highly specific and ultra-sensitive cDNA synthesis and real-time PCR in a single tube. The kits can be used to quantify any RNA template including mRNA, total RNA and viral RNA sequences. qPCRBIO SyGreen 1-Step Detect is designed for sensitivity and is ideally suited to the detection of extremely low copy number targets. qPCRBIO SyGreen 1-Step Go gives the earliest Ct and is formulated for rapid and accurate results from high template concentrations.

More Less

Product category: Polymerase chain reaction (PCR)

qPCRBIO Probe 1-Step Go

qPCRBIO Probe 1-Step Go uses the latest developments in enzyme technology and buffer chemistry to give fast, efficient cDNA synthesis and subsequent real-time PCR in a single tube. This universal probe kit is engineered for use on a wide range of probe technologies and can be used to quantify any RNA template including mRNA, total RNA and viral RNA sequences. qPCRBIO Probe 1-Step Go is designed to give rapid and accurate results over a broad range of template concentrations and is ideally suited to the detection of extremely low copy number targets.

More Less

Product category: Polymerase chain reaction (PCR)

UltraScript 2.0 cDNA Synthesis Kits

UltraScript 2.0 Reverse Transcriptase is a robust and highly thermostable modified MMLV reverse transcriptase engineered for superior cDNA synthesis speed, yield and representation from a wide range of RNA sample types, including GC-rich and low abundance templates. The enhanced thermostability of UltraScript 2.0 Reverse Transcriptase (RTase) allows reaction temperatures of over 55°C to be used, giving improved specificity, higher cDNA yields, and more full length cDNA product.

For RT-qPCR applications, the 2-tube UltraScript 2.0 cDNA Synthesis Kit contains all the components required for fast, reliable and unbiased cDNA synthesis, including an optimised blend of anchored oligo(dT) and random hexamers. UltraScript 2.0 cDNA Synthesis Kit Separate Oligos provides anchored oligo(dT) and random hexamers in separate tubes, for user optimisation depending on the type of analysis needed.

UltraScript 2.0 kits can be used with 20pg to 3.5μg total RNA or oligo(dT) purified mRNA. The RTase is pre-blended with an advanced RNase inhibitor to prevent degradation of RNA by contaminating RNase.

More Less

Product category: Polymerase chain reaction (PCR)

PCRBIO Taq DNA Polymerase

PCRBIO Taq DNA Polymerase is an affordable, versatile and robust enzyme for all your everyday PCR applications including genotyping, screening and library construction. An enhanced 12-step purification strategy together with an optimised buffer system enable PCRBIO Taq DNA Polymerase to amplify with the highest speed, yield and specificity on the market, ideal for complex templates such as mammalian genomic DNA.

For added convenience, PCRBIO Taq DNA Polymerase is available as a ready-to-use 2x mix containing all reaction components except primers and template. PCRBIO Taq Mix Red contains a red dye suitable for direct loading and tracking during agarose gel electrophoresis.

More Less

Product category: Polymerase chain reaction (PCR)

PCRBIO HiFi Polymerase

PCRBIO HiFi Polymerase is a versatile high fidelity enzyme possessing 3’-5’ exonuclease proofreading activity. Enhanced DNA binding gives inherently high processivity, increased yields and shorter cycling times while minimising PCR inhibition from impure samples such as colony and direct PCR.

More Less

Product category: Polymerase chain reaction (PCR)

PCRBIO Ultra Polymerase and Mixes

PCRBIO Ultra Polymerase has been engineered for the amplification of extremely difficult templates. Proprietary modifications that enhance processivity together with advanced buffer chemistry and hot start technology deliver outstanding performance whether your template is GC or AT rich, low in abundance or contains PCR inhibitors. The enzyme and buffer system have been developed to give superior PCR performance and higher success rates on a broad range of templates, including complex genomic DNA and targets with a high GC content (up to 80% GC). 

PCRBIO Ultra Polymerase is also available as a convenient 2x ready mix containing all reaction components except primers and template. PCRBIO Ultra Mix Red contains a preloaded red dye suitable for direct loading and tracking during agarose gel electrophoresis.

More Less

Product category: Polymerase chain reaction (PCR)

PCRBIO Rapid Extract Lysis Kit

PCRBIO Rapid Extract Lysis Kit has been developed for fast, efficient extraction of DNA from a variety of tissues and is particularly suited to solid tissue such as mouse tail or mouse ear. Sample processing is simplified and contamination risks minimised as DNA extraction is performed in a single tube, removing the need for multiple washing steps. The kit contains a lysis and protease buffer system allowing the generation of PCR-ready DNA in as little as 15 minutes, for use in downstream PCR or qPCR reactions.

More Less

Product category: Polymerase chain reaction (PCR)

PCRBIO DNA Markers

PCRBIO Ladders I-IV are designed for easy size determination and DNA quantification using agarose electrophoresis. The ladders are room temperature stable and ready for immediate gel loading. They cover a wide range (from 50bp to 10kb) and contain multiple dyes for simple migration observation. All four PCRBIO Ladders are manufactured using a combination of plasmid restriction digest fragments and PCR amplification products.

More Less

Product category: Polymerase chain reaction (PCR)

PCRBIO dNTP Mix

PCRBIO dNTP Mix contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP available at a final concentration of 10mM each or 25mM each. The mix is ultra pure (more than 99%), stable after multiple freeze-thaw cycles and perfect for a wide variety of applications including standard PCR, real-time PCR, high-fidelity PCR, 1-Step PCR and long range PCR. 

More Less

About us

Company details

PCR Biosystems is a specialist PCR company offering a range of best-in-class kits and reagents for real-time PCR, endpoint PCR and cDNA synthesis. Our reagents combine enhanced polymerases with highly developed reaction buffers and antibody-mediated hot start chemistry to maximise yield and sensitivity from the simplest to most challenging of reactions.

The PCRBIO portfolio covers a broad range of applications including real-time PCR, high fidelity PCR, hot start PCR, long PCR, PCR direct from crude samples and molecular diagnostic PCR. Comprehensive competitor comparisons show that on average we outperform all competitors in yield, sensitivity, specificity and speed, giving your reaction the best chance of working as you want it to, first time.

We offer a broad range of standard and custom solutions including bulk supply of reagents (both catalogue and custom), OEM manufacturing and expert technical support to help you achieve the most from our marketing-leading polymerases.

PCR Biosystems was founded in 2012 and has its manufacturing headquarters in London, UK. The company meets the quality standards for ISO9001:2015 and ISO13485:2016, meaning our products and processes meet the highest possible standards for reliability, efficiency and quality.

More Less

Company information as a PDF file