Cellbind, a micro column immunofixation system
The Cellbind Screen assay is a micro column test system in which sensitized red cells from a suspension are caught by a gel matrix containing anti-IgG, anti-IgM, and anti-C3d in an enhancing high-density medium.
Benefits of Cellbind
The strongest advantages of Cellbind are the flexibility and the easy readable outcome.
Cellbind Screen is intended for use in the detection or identification of red cell antibodies, as well as for blood grouping, cross-matching and the modified direct antiglobulin test (DAT, for the detection of in vivo coating of red cells with antibodies and complement components).
2. Easy to read
In positive reactions, red cells will be caght in the top layer of the gel matrix. In negative reactions, only a discrete button of red cells at the bottom of of the micro column will be seen. Reading of Cellbind cards, by eye or with a reader, is very easy and unambiguous.
3. Easy for automation
Cellbind Screen is suitable for use in manual as well as (semi-)automated systems.
The test is based on the immunofixation of sensitized red cells in a micro column containing a gel matrix. The cell suspension is added to the incubation compartment of the micro column, together with the plasma, serum or blood grouping reagent to be tested.
During the incubation phase antigen-positive red cells will bind the corresponding anti-red cell antibodies present in the plasma, serum or reagent.
Next, the cards are subjected to three phases of centrifugation:
• In the first phase optimally sensitized red cells are caught by ligands: anti-IgG, anti-IgM and anti-C3d in an enhancing High Density Medium. This results in a 3-dimensional network of red cells, attached to each other by these ligands.
• In the second phase this network is compressed into aggregates during landing on the surface of the gel.
• In the third phase non-sensitized red cells will move towards the bottom of the micro column.