PCR Biosystems Ltd. of London at MEDICA 2017 in Düsseldorf -- MEDICA Trade Fair
Manufacturers OEM

PCR Biosystems Ltd.

London Bioscience Innovation Centre, 2, Royal College Street, NW1 0NH London
United Kingdom of Great Britain and Northern Ireland

Telephone +44 207691 3563
Fax +44 207681 2186
info@pcrbio.com

This company is co-exhibitor of
The Gambica Association Limited

Hall map

MEDICA 2017 hall map (Hall 3A): stand 3AD02-4

Fairground map

MEDICA 2017 fairground map: Hall 3A

Our range of products

Product categories

  • 03  Diagnostics
  • 03.01  Clinical chemistry
  • 03.01.04  Enzymes

Our products

Product category: Enzymes

20x dUTP UDGase Additive

  • Prevents carry-over contamination
  • Highly active
Contamination PCRs with trace amounts of PCR products, called carry-over contamination, yields false positive results. Carry-over contamination from previous PCR can be a significant problem, due to the abundance of PCR products.

Carry-over contamination can be prevented by incorporating dUTP in all PCR products and treating all subsequent fully preassembled starting reactions with uracil DNA glycosylase (UDG), followed by thermal inactivation of UDG. UDG cleaves the uracil base from the phosphodiester backbone of uracil-containing DNA, but has no effect on natural (i.e., thymine-containing) DNA. The resulting apyrimidinic sites block replication by DNA polymerases, and are very labile to acid/base hydrolysis. Because UDG does not react with dUTP, and is also inactivated by heat denaturation prior to the actual PCR, carry-over contamination of PCRs can be controlled effectively if the contaminants contain uracils in place of or in addition to thymines.

20x dUTP UDGase Additive is compatible with PCR Biosystems range of real-time PCR mixes. The reagent contains dUTP, stabilizers and uracil DNA glycosylase (UDGase). The dUTP concentration ensures sufficient incorporation for complete removal of contaminating PCR products.

More Less

Product category: Enzymes

qPCRBIO SyGreen Mix

  • Non-PCR inhibiting intercalating dye
  • Available with non-reactive blue dye to assist researchers during pipetting
  • Rapid extension rate for early Ct values
  • Market leading sensitivity - increased limit of detection
  • Compatible on all real-time PCR platforms - standard and fast cycling conditions
PCR Biosystems use a proprietary intercalating dye which does not inhibit PCR, unlike other popular fluorescent dyes including SYBR® Green. Combined with advanced enzyme, hot-start and reaction buffer technology we offer market leading sensitivity and reproducibility.

qPCRBIO SyGreen Mix can be used to quantify any DNA template including genomic, cDNA and viral sequences. Extremely low copy number targets can be detected specifically with high efficiency. Proprietary small molecular inhibitor technology prevents formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity. Combining the latest advancements in polymerase technology and advanced buffer chemistry we offer market leading performance with minimal or no optimisation.

Many modern real-time PCR instruments have a fast cycling mode. The same developments that improve the sensitivity and consistency of qPCRBIO SyGreen Mix in standard cycling conditions also allow for industry leading performance in fast and ultra fast cycling conditions.

We have compared the performance of our mix and 6 competitor mixes on 4 house keeping genes, selected at random from the RTPrimerDB qPCR primer database. qPCR performance was assessed in terms of Ct values, sensitivity and efficiency. Overall, qPCRBIO SyGreen Mix outperformed all 6 competitor mixes tested. Click here to download the results of this detailed competitor comparison.

More Less

Product category: Enzymes

Next Generation Sequencing Reagents

DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. It includes any method or technology that is used to determine the order of the four bases—adenine, guanine, cytosine, and thymine—in a strand of DNA. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery.
 
Knowledge of DNA sequences has become indispensable for basic biological research, and in numerous applied fields such as medical diagnosis, biotechnology, forensic biology, virology and biological systematics. The rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the sequencing of complete DNA sequences, or genomes of numerous types and species of life, including the human genome and other complete DNA sequences of many animal, plant, and microbial species.
 
Coming Soon - qPCRBIO Library Quantification kit for Illumina Systems

The qPCRBIO Library Quantification Kit offers a simple and reliable qPCR-based method for the quantification of libraries prepared for Illumina® NGS systems. The kit contains 6 quantification standards, primers specific to the P5 and P7 Illumina® adapter sequences, qPCRBIO SyGreen Mix and an advanced buffer system optimised to give consistent library quantification across a wide range of sample types, fragment sizes (up to 1000bp), concentrations and GC content.

More Less

About us

Company details

Leading PCR Development | PCR Biosystems

PCR Biosystems offers a range of best-in-class kits and reagents for PCR and related technology. By combining our enhanced polymerases with highly developed reaction buffers and novel chemistry PCR Biosystems is leading the development of PCR. Our PCR reagents have been developed to maximise yield and sensitivity in the simplest and most challenging of reactions.

Our portfolio covers a broad range of applications including, real-time PCR, endpoint PCR, high fidelity PCR, hot start PCR, long PCR, PCR direct from crude samples and molecular diagnostic PCR.

All PCR Biosystems products have undergone comprehensive competitor product comparisons. On average we out perform all competitors in yield, specificity, sensitivity and speed. For more information on our technology click here.

PCR Biosystems Limited conforms to the Quality Management System standard ISO 9001:2008. Our certificate is valid for the scope of development, manufacture and sale of kits and reagents for scientific works in the field of molecular biology. Click here to view our certificate. 

More Less