A group of scientists at Nagoya University, Japan, have developed an incredibly versatile DNA fluorescent dye, named 'Kakshine' after a former NU student of its members, Dr. Kakishi Uno, but it also means to make the nucleus shine brightly, since the nucleus is pronounced 'Kaku' in Japanese.
Dr. Uno, with Dr .Yoshikatsu Sato and Nagisa Sugimoto, the other two members of the research team at the Institute of Transformative Bio-Molecules (ITbM), succeeded in developing a DNA binding fluorescent dye with the pyrido cyanine backbone, which satisfied the three principal qualities required of such a dye - that it has high selectivity for DNA, ability to use visible light with limited phototoxicity, and be applicable to a wide range of organisms - in a way that no previous dye has been able to.
Allowing discrimination between organelle DNA using low phototoxicity visible light, Kakshine offers easy imaging even with cutting edge microscopy techniques.
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Adding to the central set of functions required for such a chemical, Kakshine and its derivatives are highly compatible with cutting-edge microscope techniques. They represent the first dye of its kind to achieve super-resolution imaging of mitochondrial DNA in living cells with STED imaging, a kind of microscopy whose resolution exceeds the diffraction limit of light. Additionally, they also enable deep tissue imaging by two-photon excitation imaging, discrimination of different organelle DNAs with a single dye by fluorescence lifetime imaging.
Kakshine is an exceptionally versatile new dyeing agent which improves upon the capabilities of and solves the shortcomings of current-generation fluorescent dyes in DNA imaging. Moreover, with applications in the medical and life science fields including electrophoresis, quantitative PCR and flow cytometry, Kakshine is expected to make a splash as the next-generation tool for DNA analysis.
MEDICA-tradefair.com; Source: Institute of Transformative Bio-Molecules (ITbM), Nagoya University