This patented technology adds recessed micro- and nano-electrodes to the tip of an atomic force microscope (AFM), creating a single tool that can simultaneously monitor topography along with electrochemical activity at the cell surface.
The new multi-functional imaging technique will advance the study of biological samples, said Boris Mizaikoff, an associate professor at Georgia Tech's School of Chemistry and Biochemistry and director of its Applied Sensors Lab. "Conventional AFM can image surfaces, but usually provides limited chemical information," he explained. "And though scanning electrochemical microscopy (SECM), another probing technique, provides laterally resolved electrochemical data, it has limited spatial resolution. By combining AFM and SECM functionality into a single scanning probe, our tool provides researchers with a more holistic view of activities at the cell surface."
In the ATP study, which is sponsored by the National Institutes of Health and done in collaboration with Douglas Eaton at Emory University's School of Physiology, the Georgia Tech team used the multi-scanning biosensors to study ATP release at the surface of live epithelial cells. ATP is of interest to medical researchers because elevated levels have been linked with cystic fibrosis.
Using epithelial cell cultures from Emory, the Georgia Tech researchers have demonstrated that their multi-functional biosensors work at the live-cell surface during in vitro studies.
"Before you can identify what triggers the ATP release, we must be able to quantitatively measure the released species at the cell surface," Mizaikoff said. Improved understanding of cellular communication can lead to new strategies for treating diseases, he added: "There are many clinical research problems that these biosensors can help with."
MEDICA.de; Source: Georgia Institute of Technology