This study evaluated and compared the performance of two real-time PCR assays using nested reverse transcription (RT)-PCR as the reference method. To evaluate and compare the performance of two real-time PCR assays using nested RT-PCR as the reference method. Among 117 nested RT-PCR positive cases, the abTES™ DEN 5 qPCR detected 97.4% of DENV infections, while the innuDETECT Dengue TwoStep Assay did so for 44.4%. Sensitivity varied by infecting serotype and the stage of infection. The abTES™ kit has the potential to replace nested RT-PCR for rapid diagnosis of DENV infection.
J. Clin. Microbiol. October 2014 52:3781-3783; doi:10.1128/JCM.01870-14