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An Eye on Stem Cells
By means of single cell
transplantation from four different
transgenic fish lines (above) the
scientists generated a retina
with several different ArCoS;
© Heidelberg University
Stem cells help the body to grow or regenerate damaged cells or tissues. This universal response to various problems in the adult organism, even in the brain, is currently in the focus of research all over the world. One key question has so far remained unanswered: Is the “SWAT team” of stem cells a team of specialists or is it composed of individual universalists? In other words: does every cell type to be repaired require a special type of stem cells or can any single stem cell divide and generate offspring that fixes all problems in its respective environment?
To address this key question, the Heidelberg scientists combined a permanent genetic cell labelling technique with single cell transplantations in medaka fish as a model organism. Thus, the research group headed by Professor Joachim Wittbrodt could label single stem cells in the medaka retina and all of their descendants in a complete lineage tree. This allowed them to study the behaviour of single stem cells in their “natural” environment, namely the growing fish retina. The results obtained in this study demonstrate that retinal stem cells are multi-potent and a single stem cell can develop into all retinal cell types.
Experimentally this is highlighted by the formation of so-called Arched Continuous Stripes (ArCoS), labelled stripes that go through the entire retina and contain all retinal cell types. All these ArCoS originate in a single retinal stem cell. “Interestingly, different cell types within an ArCoS are more closely related than neighbouring cells of one cell type”, says Doctor Lázaro Centanin.
The results indicate that the growth of the retina is regulated by controlling stem cell proliferation, while the differentiation into specific cell types proceeds as an intrinsic programme. “It‘s as if a house was built by a universal craftsman who does it all, laying bricks, plastering, plumbing and roofing all simultaneously because the craftsman it is able to split himself up“, says Wittbrodt.
In addition, the scientists could show that in the same organ – the fish eye – a second class of stem cells exists besides the multi-potent retinal stem cells: the stem cells of the pigmented epithelium. According to Wittbrodt, the growth rates of both stem cell populations are closely correlated. The retinal pigment stem cells, however, have a limited potential and are merely unipotent.
MEDICA.de; Quelle: Heidelberg University