11/07/2008

GlysBy s.n.c.

Tetanus : The First Immunochromatographic Rapid Test by GlysBy

For qualitative detection of tetanus antitoxin antibodies
in whole blood (even fingertip capillar blood), serum or plasma samples.

Tetanus is a serious disease, leading to prolonged
hospitalisation with sequelae, and that may lead to death
(20% of the cases). This infectious disease is due to the
exotoxin produced by Clostridium tetani. This ubiquitous
Gram-positive anaerobic bacillus is occasionally present in
animal digestive tract, and persists in animal droppings and in
the ground in a very resisting sporulated form. In case of
wound, it penetrates within the body. Then, the tetanus toxin
blocks neurotransmitter release, and leads to muscular
contractures and spasms.

Since the disease confers no immunity, vaccination is the
only prevention.

Immunoprotection is proven by measuring anti-tetanus
antibody concentration. As part of emergency medicine,
knowing the real-time immune status in subjects at risk (deep
injuries) enhances better management with appropriate anti-
tetanus prophylaxis.

Detection threshold
the detection threshold of the test was
defined to 0.1 IU/mL on serum and whole blood.

Sensitivity and specificity
A clinical study was conducted in an emergency unit on 159
patients suffering from cutaneous-mucous wounds. This
study enabled to obtain 89% sensitivity on whole blood, and
94% sensitivity on serum. The obtained specificity is equal to
100%.

Precision
The negative and positive values were correctly identified in
100% of the cases.