10/05/2012

LDN Labor Diagnostika Nord GmbH & Co. KG

Multiplex Immunoassay for Catecholamines

Determination of plasma and urinary biogenic amines like catecholamines and metanephrines is generally considered as a principal test for the clinical chemical diagnosis of these diseases. Moreover, for the diagnosis of schizophrenia and bipolar affective disorders the combined analysis of biogenic amines can provide important diagnostic information. Therefore, there is great interest to measure these analytes in an efficient and cost-effective multiplex format.

LDN developed a 3-plex bead-based immunoassay for the simultaneous and quantitative determination of adrenaline, noradrenaline and dopamine in urine samples.
The read-out was performed using the Luminex FLEXMAP 3D system (Luminex Corp., Austin, TX, USA).

Advantages:

- Bead-based assay allowed simultaneous and quantitative determination of adrenaline, noradrenaline and dopamine in urine
- 3 parameters in a single assay
- simple analytical procedures
- cost-effective and time-saving
- preliminary results for a 3-plex serotonin, free metanephrine & free normetanephrine assay