Total cholesterol and HDL cholesterol visual color tests
Total and HDL Cholesterol tests are now available for home use on finger tip blood drop . The tests are extremely simple to run and does not need any instrument nor any difficult comparison with result charts.
A color is developed in minutes which is proportional to the cholesterol ( total or HDL) level in blood stream.
As example, we illustrate the Total Cholesterol Home Screening Test
The test is intended for in-vitro diagnostic for the semi-quantitative determination of Total Cholesterol in whole blood
Cholesterol measurements are used in the diagnosis and treatment of disorders involving excess cholesterol in the blood and lipid and lipoprotein metabolism disorders.
This test provides preliminary analytical results. All results indicating elevated blood cholesterol levels should be verified by a confirmatory method. Clinical considerations and professional judgment should be applied to the interpretation of results by this test.
Summary and Explanation
Cholesterol, a lipid, is associated with the pathogenesis of atherosclerosis and coronary artery disease, and the measurement of cholesterol blood levels is used to classify patients according to coronary heart disease risk, to diagnose and treat various primary or secondary hyperlipidemias, and to monitor changes resulting from treatment.
Principle of the Test
The Total Cholesterol Home Screening Test includes test strips that are treated with chemical reagents. A whole blood sample is absorbed on a receiving pad on the back of a test strip. The blood sample absorbs through to the front of a test strip and reacts with the reagents. The chemistry used is the very well established cholesterol esterase/cholesterol oxidase enzymatic reaction to produce hydrogen peroxide followed by the peroxidase catalyzed reaction hydrogen peroxide with a colorless indicator which thereby produces color. The color produced on the reaction pad can be related to the cholesterol concentration in the blood by comparison with a supplied color chart. The deeper the color is, the higher the Cholesterol level.
· If you are right-handed, you should get blood from the “middle” or “ring” finger of your left hand. Reverse if left handed.
· Wipe your finger with an alcohol swab. Let your finger hang down at your side while the alcohol dries.
· Press your fingertip with your thumbnail. Place finger flat on tabletop
· Place the hole in the bottom of the lancet against your fingertip . Press the lancet firmly against your fingertip.
Notes: It is important to pick a finger that is callous-free.
· Warning: Never use a pipette that has been used by another person!
· Squeeze blood toward your fingertip using the thumb of your other hand (push from palm toward fingertip), until you get a large drop of blood .
· Gently touch the blood drop to the tip of the pipette . The pipette will start to fill with blood, Do not pick up the pipette while it is filling.
· Fill the pipette up to the black dot. If necessary, squeeze your finger to get more blood.
· Gently pick up the pipette by the bulb (not the tip). Hold the pipette above the Test Circle. Squeeze the bulb gently to empty of the blood onto the Test Circle. Do not poke the Test Circle with the pipette tip.
· Begin timing. Wait 3 minutes before reading test result.
· After 3 minutes, turn the test strip over The Result Circle (next to the arrow with the words “Color” will be either yellow-green, green, or blue-green.
· Place the test strip behind the Color Chart so the words “Read Color” are facing you . Slide the test strip up and down so the Result Circle appears in each of the 5 windows on the Color Chart.
· Compare the color of the Result Circle to each of the 5 color blocks. Look for the best color match (the correct shade of yellow-green, green, or blue-green). Do not try to match how dark (intense) the colors are. The color of the Result Circle may be between two color blocks. Read colors under bright light, but not in direct sunlight.
· Hold the test strip so the Result Circle shows through the window of the best-matching color block. (If the color of the Result Circle is between two color blocks, hold the strip between those two blocks) The arrow on the test strip will point to a number printed on the edge of the Color Chart. THIS IS YOUR TOTAL CHOLESTEROL TEST RESULT
Results are displayed in both, milligrams per deciliter (mg/dL) and in millimoles per liter (mmol/L). The mg/dL measurement is a US version, while mmol/L is used in Europe and many other countries around the world.
Hint: Look carefully for any blue shading in the Result Circle. For example, if the “Result Circle” has some blue in it, but not as much blue as the “275” color block, hold the test strip between the “200” and “275” color blocks. The arrow will point to a Total Cholesterol test result of 240 mg/dL . If you are not sure about the best color match, look at the Result Circle through the lowest of the 5 windows. The yellow-green background of this color block may help you see any blues or greens in the Result Circle.
UNDERSTANDING YOUR TEST RESULT
Your test result will place you in one of 3 medical risk groups defined by the National Cholesterol Education Program:
Result less than 200 mg/dL (5.2 mmol/L): “Desirable” Group
You are most likely at low risk for heart disease. If you have other risk factors for heart disease, you should talk with your doctor. If you have no other risk factors, you should recheck your total cholesterol level every 6 months.
Result between 200-239 mg/dL (5.2 — 6.2 mmol/L): “Borderline-High” Group
You may be at increased risk for heart disease. You should talk with your doctor.
Result 240 mg/dL (6.2 mmol/L) or above: “High” Group
You may be at increased risk for heart disease, whether or not you have additional risk factors. You should ask your doctor for advice.